{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:15:21Z","timestamp":1789758921254,"version":"4.0.1"},"reference-count":0,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[1993,1,1]],"date-time":"1993-01-01T00:00:00Z","timestamp":725846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1993,1,1]],"date-time":"1993-01-01T00:00:00Z","timestamp":725846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Kidney Blood Press Res"],"published-print":{"date-parts":[[1993]]},"abstract":"The mechanisms responsible for transepithelial Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> transport in the isolated perfused cortical thick ascending limb (cTAL) of Henle\u2019s loop of the mouse nephron were investigated by measuring transepithelial voltages (PD<sub>te<\/sub>) and transepithelial ion net fluxes (J<sub>Na<\/sub>, J<sub>c1<\/sub>, J<sub>\u0138<\/sub>, J<sub>ca<\/sub>, J<sub>Mg<\/sub>) by electron microprobe analysis. In the presence of furosemide (10<sup>-4<\/sup> mol\u00b7l<sup>-1<\/sup>, lumen) and diphenylamine-2-carboxylate (DPC, 10<sup>-4<\/sup> mol\u00b7l<sup>-1<\/sup>, bath), known inhibitors of NaCl reabsorption in the TAL, Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> reabsorption was completely inhibited. In the presence of furosemide, J<sub>Ca<\/sub> fell from 0.75 \u00b1 0.07 to -0.08 \u00b1 0.09 pmol\u00b7min<sup>-1<\/sup>.mm<sup>-1<\/sup> (n = 5) and J<sub>Mg<\/sub> from 0.47 \u00b1 0.04 to -0.01 \u00b1 0.11 pmol\u00b7min<sup>-1<\/sup>.mm<sup>-1<\/sup> (n = 5). In the presence of DPC, J<sub>Ca<\/sub> fell from 0.57 \u00b1 0.08 to -0.07 \u00b1 0.11 pmol\u00b7min<sup>-1<\/sup>.mm<sup>-1<\/sup> (n = 5), and J<sub>Mg<\/sub> from 0.16 \u00b1 0.02 to-0.11 \u00b1 0.07pmol\u00b7min<sup>-1<\/sup>\u00b7mm<sup>-1<\/sup>(n = 5). With furosemide, inhibition of Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> transport was paralleled by a 93% inhibition of NaCl reabsorption, while in the presence of DPC there was a 60% reduction of NaCl reabsorption. These effects were fully reversed after removal of the inhibitors from the lumen or bath solutions. In the absence of active NaCl transport, a lumen-to-bath directed-NaCl gradient (lumen: 150 m<i>M<\/i> NaCl + furosemide, bath: 50 m<i>M<\/i> NaCl + 200 m<i>M<\/i> mannitol) generated a negative transepithelial dilution potential of-13.8 \u00b1 1.1 mV (n = 8) which induced a significant Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> secretion into the tubular lumen of -0.59 \u00b1 0.06 and 0.43 \u00b1 0.05pmol\u00b7min<sup>-1<\/sup>\u00b7mm<sup>-1<\/sup> (n = 8), respectively. Abath-to-lumen-directed NaCl gradient on the other hand (lumen: 50 m<i>M<\/i> NaCl + furosemide, bath: 150m<i>M<\/i> NaCl) generated a positive transepithelial dilution potential of +15.9 \u00b1 0.6 mV (n = 7), inducing a significant Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> reabsorption of 0.62 \u00b1 0.08 and 0.38 \u00b1 0.07 pmol\u00b7min<sup>-1<\/sup> mm<sup>-1<\/sup>(n = 7), respectively. Linear regression analysis of individual Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> net flux data versus voltage indicated that J<sub>Ca<\/sub> and J<sub>Mg<\/sub> were highly correlated to PD<sub>te<\/sub>. In conclusion, these data indicate that transepithelial Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> reabsorption in the mouse cTAL is predominantly a passive process, driven by the lumen-positive PD<sub>te<\/sub>.<\/jats:p>","DOI":"10.1159\/000173762","type":"journal-article","created":{"date-parts":[[2008,11,12]],"date-time":"2008-11-12T13:17:04Z","timestamp":1226495824000},"page":"157-166","source":"Crossref","is-referenced-by-count":11,"title":["Transepithelial Ca<sup>2+<\/sup> and Mg<sup>2+<\/sup> Transport in the Cortical Thick Ascending Limb of Henle\u2019s Loop of the Mouse Is a Voltage-Dependent Process"],"prefix":"10.1159","volume":"16","author":[{"given":"A.","family":"Di Stefano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Roinel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"de Rouffignac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Wittner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2008,11,11]]},"container-title":["Kidney and Blood Pressure Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/173762","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/173762","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:38:04Z","timestamp":1745455084000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000173762"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2004,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000173762","archive":["Portico"],"relation":{},"ISSN":["1420-4096","1423-0143"],"issn-type":[{"value":"1420-4096","type":"print"},{"value":"1423-0143","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993]]}}}