{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T15:59:49Z","timestamp":1779379189051,"version":"3.53.1"},"reference-count":23,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2008,11,26]],"date-time":"2008-11-26T00:00:00Z","timestamp":1227657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,11,26]],"date-time":"2008-11-26T00:00:00Z","timestamp":1227657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2009]]},"abstract":"<i>Background:<\/i><i>Blomia tropicalis<\/i> is an important domestic dust mite in the tropics and subtropics. This study describes cDNA cloning of the group 4 allergen of <i>B. tropicalis<\/i>, and the evaluation of the sensitization of this allergen in atopic populations from 2 geographic regions. <i>Methods:<\/i> cDNA cloning was carried out using the Smart RACE cDNA amplification kit. The full-length Blo t 4 cDNA was isolated by cDNA library screening, 5\u2032 and 3\u2032 rapid amplification of cDNA ends and long-distance PCR. Sequence analysis was performed with a combination of the Clustal W, CGC and Blast program packages. The cDNA was expressed in <i>Pichia pastoris<\/i> yeast. The skin prick test was used to evaluate the sensitization profile of recombinant Blo t 4, crude dust mite allergen extracts and major <i>B. tropicalis<\/i> recombinant allergen Blo t 5. <i>Results:<\/i> The cloned Blo t 4 had a molecular weight of 56 kDa and had 68% amino acid homology with group 4 allergens of <i>Dermatophagoides pteronyssinus<\/i> and 65% with those of <i>Euroglyphus maynei<\/i>. A sensitization profile to the expressed recombinant Blo t 4 allergen (28%) showed an unusually higher frequency than to the major allergen Blo t 5 (22%) in allergic subjects from Chengdu, PR China. In comparison, the subjects from Singapore showed very low sensitization to Blo t 4 (4%) compared with Blo t 5 (84%). <i>Conclusions:<\/i> Group 4 allergens of <i>B. tropicalis<\/i> may be an important dust mite allergen in certain distinct populations.<\/jats:p>","DOI":"10.1159\/000176303","type":"journal-article","created":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T13:16:09Z","timestamp":1228137369000},"page":"25-32","source":"Crossref","is-referenced-by-count":23,"title":["Mite Amylase from <i>Blomia tropicalis<\/i> (Blo t 4): Differential Allergenicity Linked to Geographical Regions"],"prefix":"10.1159","volume":"149","author":[{"given":"Nge","family":"Cheong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Donnie A.","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chwee Ying","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiok Hee","family":"Chng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bee Wah","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaw Yan","family":"Chua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2008,11,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-6749%2891%2992147-S"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0079-6107%2897%2900015-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.1994.tb00952.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1398-9995.1998.tb03931.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2F0190-9622%2893%2970237-N"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159%2F000024227"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1432-1033.1994.00519.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159%2F000049525"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2003.00033.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1093%2Fprotein%2F12.10.819"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-5793%2895%2901309-1"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-1119%2800%2900229-8"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159%2F000237478"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2896%2970091-X"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-6749%2894%2990117-1"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.170.4.1457"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1093%2Fnar%2F14.11.4683"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-2836%2883%2980341-6"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2003.00215.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1159%2F000074302"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2003.167"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2802%2981667-0"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2007.02786.x"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/176303","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/176303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:24:08Z","timestamp":1745454248000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000176303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11,26]]},"references-count":23,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2009,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000176303","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,11,26]]}}}