{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:38:09Z","timestamp":1777480689534,"version":"3.51.4"},"reference-count":19,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2008,11,26]],"date-time":"2008-11-26T00:00:00Z","timestamp":1227657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,11,26]],"date-time":"2008-11-26T00:00:00Z","timestamp":1227657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2009]]},"abstract":"<i>Background:<\/i> The aspirin provocation test, considered to be the gold standard in the diagnosis of aspirin sensitivity, may be associated with severe adverse reactions; thus, alternative procedures with a higher safety profile are highly desirable. Although the cellular antigen stimulation test (CAST) has been proposed to be such an alternative, there is limited information about its clinical usefulness. <i>Objective:<\/i> It was the aim of our study to evaluate the clinical usefulness of CAST in the diagnosis of aspirin sensitivity. <i>Material and Methods:<\/i> Patients with aspirin-sensitive asthma and\/or nasal polyps (n = 40), patients with aspirin-tolerant asthma and\/or nasal polyps (n = 13) and healthy volunteers (n = 26) were included. A 2-day, single-blind placebo-controlled oral aspirin provocation test was performed. In vitro release of cysteinyl leukotrienes (Cys-LTs) by peripheral blood leukocytes was measured after stimulation with both stimulation buffer and lysine aspirin (2.5 mg\/ml) by CAST. <i>Results:<\/i> Baseline Cys-LT levels were similar among the 3 groups. After lysine aspirin stimulation, the net increase in Cys-LTs was significantly higher in patients with aspirin sensitivity (median 91 pg\/ml, interquartile range 22\u2013206) compared with aspirin-tolerant patients (20 pg\/ml, range 0\u201346) and healthy controls (23 pg\/ml, range 0\u201371; p = 0.004). The assay had a sensitivity of 25%, a specificity of 92.3%, and positive and negative predictive values of 28.7 and 90.7%, respectively. <i>Conclusion:<\/i> Although the leukocytes of patients with aspirin sensitivity produce higher amounts of Cys-LTs as measured by CAST, the low sensitivity and predictive values limit the clinical usefulness of this test in the diagnosis of aspirin sensitivity.<\/jats:p>","DOI":"10.1159\/000176307","type":"journal-article","created":{"date-parts":[[2008,11,29]],"date-time":"2008-11-29T14:18:13Z","timestamp":1227968293000},"page":"58-64","source":"Crossref","is-referenced-by-count":14,"title":["Cellular Allergen Stimulation Test with Acetylsalicylic Acid-Lysine Is Not a Useful Test to Discriminate between Asthmatic Patients with and without Acetylsalicylic Acid Sensitivity"],"prefix":"10.1159","volume":"149","author":[{"given":"Sevim","family":"Bavbek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Berna","family":"Dursun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esra","family":"Birben","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6mer","family":"Kalayc\u0131","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeynep","family":"M\u0131s\u0131rl\u0131gil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,11,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0889-8561%2805%2970038-2"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007%2Fs100670070024"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2001.01074.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2899%2970324-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2006.07.024"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159%2F000236259"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1398-9995.1996.tb00168.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2000.00953.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000082586"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1399-3003.2000.15e09.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS1081-1206%2810%2962453-2"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1398-9995.2001.00103.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02032571"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.1999.00655.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2001.00965.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1159%2F000237695"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2896%2980866-9"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1136%2Fthorax.55.suppl_2.S70"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.2174%2F138161206778193971"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/176307","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/176307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:24:09Z","timestamp":1745454249000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000176307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11,26]]},"references-count":19,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2009,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000176307","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,11,26]]}}}