{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:13:20Z","timestamp":1777907600655,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,11,25]],"date-time":"2008-11-25T00:00:00Z","timestamp":1227571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,11,25]],"date-time":"2008-11-25T00:00:00Z","timestamp":1227571200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"abstract":"<jats:p>Intravital video microscopy was used to test superoxide dismutase and a lazaroid analogue, U-74389F, as a pretreatment for ischemia-reperfusion-induced microvascular dysfunction in skeletal muscle. Twenty-two male Wistar rats (350-400 g), anesthetized with sodium pentobarbital (65 mg\/kg i.p.), were divided into groups to test the lazaroid analogue U-74389F (3 mg\/kg; n = 8), a citric acid\/citrate mixture (CS-4; n = 4) used as the vehicle for the lazaroid analogue, superoxide dismutase (SOD, 10 mg\/kg; n = 5), and saline (n = 5). Normothermic ischemia of the extensor digitorum longus muscle was induced for 3 h by tightening a tourniquet placed around the limb above the muscle. Measurements of the number of perfused capillaries (CD&lt;sub&gt;per&lt;\/sub&gt;; mm-1) and capillary red blood cell velocity (V&lt;sub&gt;RBC&lt;\/sub&gt;; mm\/s) were made after 30,60 and 90 min of reperfusion. Thirty minutes following release of the tourniquet, all test groups showed a significant drop in CD&lt;sub&gt;per&lt;\/sub&gt;. The extent of this reduction was maximal in SOD treated muscles, while it was minimized in the lazaroid-treated muscles following 90 min reperfusion. Hyperemia occurred only in muscles treated with saline or lazaroid. The hyperemia was of limited duration in saline-treated muscles, but lasted the entire reperfusion period following lazaroid treatment. An index of microvascular flow, estimated from the product of V&lt;sub&gt;RBC&lt;\/sub&gt; and CD&lt;sub&gt;per&lt;\/sub&gt;, indicated that flow was significantly greater in muscles treated with lazaroids as compared with all other groups following the 90-min reperfusion. We conclude that whereas SOD was detrimental, the lazaroid analogue U-74389F improved microvascular perfusion following 3 h of no-flow ischemia and 90 min reperfusion.<\/jats:p>","DOI":"10.1159\/000178849","type":"journal-article","created":{"date-parts":[[2008,11,29]],"date-time":"2008-11-29T09:18:13Z","timestamp":1227950293000},"page":"313-318","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Superoxide Dismutase and 21-Aminosteroids (Lazaroids) on Microvascular Perfusion following Ischemia-Reperfusion in Skeletal Muscle"],"prefix":"10.1159","author":[{"given":"R.F.","family":"Potter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.G.","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Tyml","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.C.","family":"Groom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,11,25]]},"container-title":["Journal of Vascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/178849","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000178849","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:39:55Z","timestamp":1777642795000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000178849"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11,25]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000178849","archive":["Portico"],"relation":{},"ISSN":["1018-1172","1423-0135"],"issn-type":[{"value":"1018-1172","type":"print"},{"value":"1423-0135","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,11,25]]}}}