{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:06:16Z","timestamp":1777917976599,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T00:00:00Z","timestamp":1229558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T00:00:00Z","timestamp":1229558400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"abstract":"<jats:p>We investigated patients affected by Bartter\u2019s syndrome in the attempt to localize the intracellular defect mediating the reduced intracellular Ca2+ mobilization that may be responsible for the decreased vascular reactivity characteristic of Bartter\u2019s syndrome. Using the formylmethionyl-leucyl-phenylalanine (fMLP) receptor system, which causes intracellular calcium release, we investigated fMLP-stimulated intracellular inositol 1,4,5-trisphosphate (IP3) production as well as the number and affinity of fMLP receptors in neutrophils from Bartter\u2019s syndrome patients and healthy controls. Scatchard plot analysis of radioactive fMLP binding to neutrophils indicated that there were no differences in either cell receptor number and affinity for ligand between healthy controls (n = 5) and patients with Bartter\u2019s syndrome (n = 5): 6,151 \u00b1 1,431 vs. 7,112 \u00b1 2,566 receptors\/cell; K&lt;sub&gt;D&lt;\/sub&gt;: 0.446 \u00b1 0.14 vs. 0.454 \u00b1 0.09 pM of fMLP. 5- and 10-second fMLP-stimulated intracellular IP&lt;sub&gt;3&lt;\/sub&gt; production was instead reduced in patients affected by Bartter\u2019s syndrome: 2.479 \u00b1 1.07 vs. 4.073 \u00b1 1.04nmol\/l07 cells at 5 s(n = 8;p &lt; 0.01);1.673 \u00b1 0.741 vs. 3.766 \u00b1 1.348 nmol\/l07 cells at 10 s (n \u2013 8; p &lt; 0.005). The results of this study indicate that the anomaly of intracellular calcium mobilization in patients with Bartter\u2019s syndrome arises from a defect at the postreceptor level. The anomalous calcium signalling that takes place in Bartter\u2019s syndrome may provide a mechanism for the hyporesponsiveness to pressor stimuli characteristic of these patients.<\/jats:p>","DOI":"10.1159\/000188941","type":"journal-article","created":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T09:00:08Z","timestamp":1230109208000},"page":"570-573","source":"Crossref","is-referenced-by-count":22,"title":["Intracellular Calcium Signalling and Vascular Reactivity in Bartter\u2019s Syndrome"],"prefix":"10.1159","author":[{"given":"Lorenzo","family":"Cal\u00f2","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"D\u2019Angelo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Cantaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Rizzolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Favaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Augusto","family":"Antonello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arturo","family":"Borsatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2008,12,18]]},"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/188941","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000188941","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:28:00Z","timestamp":1777660080000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000188941"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,18]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000188941","archive":["Portico"],"relation":{},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12,18]]}}}