{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T03:47:15Z","timestamp":1777866435293,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,2,5]],"date-time":"2009-02-05T00:00:00Z","timestamp":1233792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,2,5]],"date-time":"2009-02-05T00:00:00Z","timestamp":1233792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"abstract":"<jats:p>The functional role of the cholinergic nervous system in regulating gastrin release was investigated using enriched canine antral G cells. Gastrin content was 30.1 \u00b1 2.9 pmol per well and basal gastrin release was 900 \u00b1 27 fmol per well (n = 45). Carbachol (10-8 to 10-5M) dose-dependently stimulated gastrin release with a maximal stimulatory response achieved at a concentration of 10-5M (330% over basal). To characterize the muscarinic receptor which mediates gastrin release from antral G cells, we examined the effect of three muscarinic receptor antagonists on carbachol-stimulated gastrm release; atropine (nonselective muscarinic receptor antagonist), pirenzepine (M&lt;sub&gt;1&lt;\/sub&gt; muscarinic receptor antagonist) and 4-DAMP (M3 muscarinic receptor antagonist). Atropine (10-9 to 10-6M), pirenzepine (10-8 to 10-5M) and 4-DAMP (10-9 to 10-6 M) had no effect on the basal gastrin release. However, carbachol (10-5 M)-stimulated gastrin release was effectively inhibited by atropine and 4-DAMP with K&lt;sub&gt;i&lt;\/sub&gt; values of 0.48 and 0.66 nM, respectively. Pirenzepine at a high concentration (10-5M) also inhibited carbachol-stimulated gastrin release with a K&lt;sub&gt;i&lt;\/sub&gt; value of 46.3 nM. These results suggest that the cholinergic nervous system directly stimulates gastrin release via M&lt;sub&gt;3&lt;\/sub&gt; muscarinic receptors located on antral G cells.<\/jats:p>","DOI":"10.1159\/000201218","type":"journal-article","created":{"date-parts":[[2009,2,7]],"date-time":"2009-02-07T01:50:30Z","timestamp":1233971430000},"page":"31-34","source":"Crossref","is-referenced-by-count":15,"title":["Muscarinic M\n                    <sub>3<\/sub>\n                    Receptor-Mediated Release of Gastrin from Canine Antral G Cells in Primary Culture"],"prefix":"10.1159","author":[{"given":"Kunihiko","family":"Yokotani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"DelValle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadataka","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,2,5]]},"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/201218","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000201218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T08:41:28Z","timestamp":1777538488000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000201218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000201218","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"value":"0012-2823","type":"print"},{"value":"1421-9867","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2,5]]}}}