{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:37:41Z","timestamp":1767973061841,"version":"3.49.0"},"reference-count":24,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2008]]},"abstract":"<jats:p>&lt;i&gt;Objective:&lt;\/i&gt; The aim of this investigation was to study the effect of vitamin E treatment in oxidative stress of red and white cells of \u03b2-thalassaemia intermedia patients. &lt;i&gt;Methods:&lt;\/i&gt; Nine patients undergoing occasional transfusions (5 females\/4 males), median age 39 years (range 15\u201374), were recruited for oral daily administration of 400 IU vitamin E for 3 months. Twenty-seven milliliters of peripheral blood was obtained before and after 3 months of treatment, and 3 months after treatment completion. In the case of transfused patients (n = 4), blood was obtained at least 30 days after transfusion. Reactive oxygen species (ROS) was measured by flow cytometry; red blood cell (RBC) reduced glutathione (GSH) was measured by dinitrothiocyanobenzene reduction, serum malondialdehyde was measured in terms of thiobarbituric acid-reactive substances (TBARS), and \u03b1-haemoglobin-stabilizing protein (AHSP&lt;i&gt;) &lt;\/i&gt;mRNA expression was measured by real-time polymerase chain reaction of reticulocyte RNA extracts. &lt;i&gt;Results:&lt;\/i&gt; \u03b2-Thalassaemia patients presented basal levels of RBC ROS, GSH and serum TBARS statistically different compared with healthy controls. However, after vitamin E administration, patients presented a significant reduction in erythrocyte RBC ROS and serum TBARS levels. In parallel, red cell GSH was significantly increased after treatment. Peripheral mononuclear cells and T lymphocytes also demonstrated a reduction in ROS production. Therefore, after treatment, no significant differences were detected comparing patients and normal controls. Three months after treatment completion, all measurements showed a tendency of returning to basal values. A significant reduction in reticulocyte number was observed after vitamin E treatment. Vitamin E treatment did not modify levels of haemoglobin or &lt;i&gt;AHSP &lt;\/i&gt;mRNA expression. &lt;i&gt;Conclusion:&lt;\/i&gt; Although vitamin E is not capable of reducing anaemia in these patients, it could be useful for reducing oxidative damage in other target organs of \u03b2-thalassaemic patients. Finally, this is the first study to analyse the effects of vitamin E on ROS production in red and white blood cells and &lt;i&gt;AHSP&lt;\/i&gt; mRNA expression.<\/jats:p>","DOI":"10.1159\/000201988","type":"journal-article","created":{"date-parts":[[2009,2,16]],"date-time":"2009-02-16T14:57:42Z","timestamp":1234796262000},"page":"225-231","source":"Crossref","is-referenced-by-count":30,"title":["Vitamin E Supplementation Reduces Oxidative Stress in Beta Thalassaemia Intermedia"],"prefix":"10.1159","volume":"120","author":[{"given":"W.P.","family":"Pfeifer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.R.","family":"Degasperi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.T.","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.E.","family":"Vercesi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.F.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.T.O.","family":"Saad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,2,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1537-2995.2007.01192.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM199907083410207"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.1995.tb05394.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI31664"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature00803"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-0609.2003.00011.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.2007.06654.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS1040-8428%2899%2900058-X"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0021-9150%2897%2900300-6"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.2005.05463.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.2005.05834.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0300-483X%2803%2900159-8"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1440-1843.2003.00534.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002%2Fcyto.a.20017"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2003-09-3167"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI32362"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1196%2Fannals.1345.014"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002%2Fajh.21041"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.2006.06082.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.intimp.2006.04.004"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.freeradbiomed.2004.09.017"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.freeradbiomed.2004.10.024"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.freeradbiomed.2007.03.024"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1093%2Fcarcin%2F18.2.359"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/201988","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/201988","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T23:57:16Z","timestamp":1745452636000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000201988"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":24,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000201988","archive":["Portico"],"relation":{},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"value":"0001-5792","type":"print"},{"value":"1421-9662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008]]}}}