{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:41:14Z","timestamp":1790872874724,"version":"4.1.0"},"reference-count":19,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Nutr Metab"],"published-print":{"date-parts":[[2009]]},"abstract":"<i>Background:<\/i> Recent studies in animal models have shown that high methionine intakes induce atherosclerotic changes that may be exacerbated when deficiencies of vitamins B<sub>6<\/sub>, B<sub>12<\/sub> and folate are present. However, the mechanism underlying this possible atherogenic effect remains unknown. The aim of the present study was to evaluate the effects of methionine on the folate-dependent thymidylate-DNA synthesis, as a possible mechanism of atherogenicity, as well as the effect of high methionine\/low folate on several key inflammation markers, such as vascular cell adhesion molecule-1 (VCAM-1), receptor for advanced glycation end products (RAGE) and matrix metalloproteinase-9 (MMP-9) in human aortic endothelial cells. <i>Methods:<\/i> Deoxyuridine suppression test was performed in order to evaluate thymidylate synthesis. To examine the expression of inflammation markers, cells were exposed to high methionine\/low folate media for 9 days. <i>Results:<\/i> The assayed methionine levels (0.1, 0.5 and 5 m<i>M<\/i>) did not affect the de novo thymidylate-DNA synthesis. Consistent with this result, methionine (1 and 2.5 m<i>M<\/i>), alone or in combination with folate deficiency, increased homocysteine levels but did not induce the expression of the inflammation markers evaluated. <i>Conclusion:<\/i> Under the study conditions, methionine was not able to exert the atherogenic mechanism proposed and did not have the hypothesized inflammatory consequences in human aortic endothelial cells.<\/jats:p>","DOI":"10.1159\/000205317","type":"journal-article","created":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T09:37:06Z","timestamp":1235727426000},"page":"28-34","source":"Crossref","is-referenced-by-count":3,"title":["Mild Methionine Excess Does Not Affect Thymidylate Synthesis or Inflammation Markers Expression in Human Aortic Endothelial Cells"],"prefix":"10.1159","volume":"54","author":[{"given":"Mar\u00eda","family":"Ach\u00f3n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Alonso-Aperte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregorio","family":"Varela-Moreiras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Selhub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2009,2,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1001%2Fjama.288.16.2015"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.1975.tb00863.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1515%2FCCLM.2005.201"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0021-9150%2895%2905740-4"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1042%2FCS19980064"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0021-9150%2803%2900138-2"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI10588"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1161%2F01.ATV.0000035404.18281.37"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ijcard.2005.07.006"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.2436385100"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM199901143400207"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1161%2F01.ATV.0000014221.30108.08"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.2174%2F138161207783018608"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1161%2F01.ATV.0000100562.63144.C1"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0378-4347%2887%2980438-3"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.2460%2Fajvr.2002.63.1359"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200114002"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1161%2F01.RES.0000087642.01082.E4"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpcell.00207.2007"}],"container-title":["Annals of Nutrition and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/205317","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/205317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T23:16:28Z","timestamp":1745450188000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000205317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":19,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2009,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000205317","archive":["Portico"],"relation":{},"ISSN":["0250-6807","1421-9697"],"issn-type":[{"value":"0250-6807","type":"print"},{"value":"1421-9697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}