{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T12:37:32Z","timestamp":1784810252068,"version":"3.55.0"},"reference-count":25,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2009]]},"abstract":"<i>Background:<\/i> Prostaglandin (PG) E<sub>2<\/sub> is thought to exert protective effects in the lungs. Accordingly, aerosolized PGE<sub>2<\/sub> prevents the experimentally induced airway response to allergen challenge in asthmatics. In vitro evidence indicating that functional PGE<sub>2<\/sub> receptors (EP) are expressed on human mast cells and that PGE<sub>2<\/sub> can alter cytokine production suggests that these phenomena may be involved in its beneficial effect in asthma. However, in vivo evidence is scarce. <i>Methods:<\/i> We assessed the effects of exogenous PGE<sub>2<\/sub> and of the EP1\/EP3 agonist sulprostone on the murine airway response to house dust mite (HDM) allergens, a model that accurately reproduces the spontaneous exposure of allergic asthma patients to aeroallergens. We also analyzed the in vivo impact of PGE<sub>2<\/sub> on production in the murine airway of mast cell protease (mMCP)-1, a specific marker of lung mast cell activity, and on local production of cytokines. <i>Results:<\/i> Exogenous PGE<sub>2<\/sub>, but not sulprostone, reduced eosinophilic infiltration in HDM-sensitized mice by half and led to a strong reduction in airway Th<sub>2<\/sub> cytokine expression. These anti- inflammatory effects were accompanied in vivo by a substantial reduction in HDM-induced upregulation of airway mMCP-1. Neither PGE<sub>2<\/sub> nor sulprostone had any effect on airway hyperresponsiveness to methacholine. <i>Conclusions:<\/i> Our results indicate that the anti-inflammatory effect of PGE<sub>2<\/sub> can be reproduced in vivo in HDM-sensitized mice and suggest that this protective effect is dependent in vivo on inhibition of the allergen-triggered proinflammatory activity of bronchial mast cells. Finally, the effect of PGE<sub>2<\/sub> is linked to reduced upregulation of airway Th<sub>2<\/sub> cytokines.<\/jats:p>","DOI":"10.1159\/000205578","type":"journal-article","created":{"date-parts":[[2009,3,18]],"date-time":"2009-03-18T14:14:15Z","timestamp":1237385655000},"page":"323-332","source":"Crossref","is-referenced-by-count":23,"title":["Subcutaneous Prostaglandin E<sub>2<\/sub> Restrains Airway Mast Cell Activity in vivo and Reduces Lung Eosinophilia and Th<sub>2<\/sub> Cytokine Overproduction in House Dust Mite-Sensitive Mice"],"prefix":"10.1159","volume":"149","author":[{"given":"Aida","family":"Herrerias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariona","family":"Serra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Marco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Roca-Ferrer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar","family":"Picado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"de Mora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2009,3,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200401-033OC"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.it.2003.11.001"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2567.2006.02376.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-6980%2802%2900065-5"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fni1188"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0706150"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI6890"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2005-07-2772"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1398-9995.2006.01043.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0706664"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2007.02820.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200308-1094OC"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2007.02.046"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI25702"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1159%2F000107054"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-6980%2801%2900186-1"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bone.2003.09.008"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1248%2Fbpb.29.1148"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002%2Feji.200535268"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2895%2970039-0"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2000.105219"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood.V98.6.1942"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2003.01827.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI31546"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200312-1651OC"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/205578","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/205578","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T23:19:02Z","timestamp":1745450342000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000205578"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":25,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000205578","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}