{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:29:11Z","timestamp":1791379751254,"version":"4.3.3"},"reference-count":26,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2009]]},"abstract":"<i>Objective:<\/i> To strengthen the role of high-risk human papillomaviruses (HPVs) in the development of cervical cancer, and the association between HPV and the cell cycle inhibitor gene p16<sup>ink4a<\/sup> in cervical carcinogenesis. <i>Methods:<\/i> In this study, the association between p16<sup>ink4a<\/sup> and the presence of HPV DNA in cervical lesions was investigated in a total of 177 cervical biopsies classified as benign (n = 42), low-grade squamous intraepithelial lesions (LSIL; n = 34), high-grade squamous intraepithelial lesions (HSIL; n = 48) and invasive cancer (n = 53). p16<sup>ink4a<\/sup> expression was analyzed by immunohistochemistry while HPV DNA was localized by in situ hybridization. <i>Results:<\/i> No significant association was found between p16<sup>ink4a<\/sup> expression and HPV presence in benign and LSIL samples. In HSIL and invasive cancer, a significant association was found between p16<sup>ink4a<\/sup> overexpression and the presence of HPV DNA. <i>Conclusion:<\/i> Immunohistochemical p16<sup>ink4a<\/sup> expression is associated with HPV infection in HSIL and cervical cancer, suggesting a role of p16 as a biomarker of HPV-associated cervical lesions.<\/jats:p>","DOI":"10.1159\/000209392","type":"journal-article","created":{"date-parts":[[2009,5,20]],"date-time":"2009-05-20T12:26:46Z","timestamp":1242822406000},"page":"141-148","source":"Crossref","is-referenced-by-count":19,"title":["p16<sup>ink4a<\/sup> Is a Surrogate Marker for High-Risk and Malignant Cervical Lesions in the Presence of Human Papillomavirus"],"prefix":"10.1159","volume":"76","author":[{"given":"S.","family":"Lakshmi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Rema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thara","family":"Somanathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2009,5,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2807%2961416-0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1042%2FCS20050369"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1204860"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.56.1.56"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.1174"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9440%2810%2965689-1"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3322%2Fcanjclin.55.2.74"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0959-8049%2802%2900462-8"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1006%2Fexcr.2000.5149"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1994.2090"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038%2Fng0994-27"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-8258%2803%2900010-6"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1097%2F00005537-200203000-00013"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1202232"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1440-1827.2002.01359.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1093%2Fjnci%2F92.9.690"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.44.12.990"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.45.5.382"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1001%2Fjama.281.17.1605"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.51.2.96"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038%2Fmodpathol.3880375"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291097-0215%2819960208%2965%3A4%3C442%3A%3AAID-IJC8%3E3.0.CO%3B2-X"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1097%2F00000478-200107000-00006"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1369%2Fjhc.4A6312.2005"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1097%2F01.pas.0000155164.78785.c2"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1309%2F0UP5PJK9RYF7BPHM"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/209392","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/209392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T22:41:04Z","timestamp":1745448064000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000209392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000209392","archive":["Portico"],"relation":{},"ISSN":["1015-2008","1423-0291"],"issn-type":[{"value":"1015-2008","type":"print"},{"value":"1423-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}