{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:04:36Z","timestamp":1761843876203,"version":"3.40.4"},"reference-count":33,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Atopic eczema is a chronic inflammatory skin disease in which several subgroups of cases can be identified. Atopy patch testing (APT) reveals allergen sensitization also in atopic eczema patients devoid of detectable allergen-specific IgE, suggesting the importance of factors other than IgE in the reaction. Here we investigate the relationship between APT reactions and allergen-specific peripheral IgE and T cell reactivity in atopic eczema patients. &lt;i&gt;Methods:&lt;\/i&gt; Adult patients with atopic eczema (n = 64) and healthy controls (n = 24) were analyzed for reactivity to &lt;i&gt;Malassezia sympodialis&lt;\/i&gt; extract by APT, measurement of specific plasma IgE and in vitro determination of the frequency of allergen-reactive peripheral blood mononuclear cells producing interleukin-4 and interleukin-5 using the ELISpot method. &lt;i&gt;Results:&lt;\/i&gt; When combining the results of the APT, IgE measurements and the ELISpot analyses, reactivity to &lt;i&gt;M. sympodialis&lt;\/i&gt; was found in a majority of the atopic eczema patients (69%), whereas the healthy controls were negative throughout. T cell reactivity to &lt;i&gt;M. sympodialis&lt;\/i&gt;, manifested by production of both interleukins 4 and 5, was highly predictive for a positive APT reaction and displayed a strongly positive correlation with the APT score. In contrast, the allergen-specific IgE levels did not predict the APT outcome, and no correlation could be found between the IgE levels and the APT score. &lt;i&gt;Conclusion:&lt;\/i&gt; Peripheral allergen-specific T helper 2 cell-mediated reactivity appears to be required for a positive APT reaction to &lt;i&gt;M. sympodialis&lt;\/i&gt;. The diagnostic potential of measuring peripheral allergen-specific T cell responses should be considered in atopic eczema.<\/jats:p>","DOI":"10.1159\/000210380","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T14:14:27Z","timestamp":1238681667000},"page":"51-58","source":"Crossref","is-referenced-by-count":16,"title":["Elevated Peripheral Allergen-Specific T Cell Response Is Crucial for a Positive Atopy Patch Test 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