{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T09:31:42Z","timestamp":1777887102811,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,4,8]],"date-time":"2009-04-08T00:00:00Z","timestamp":1239148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,4,8]],"date-time":"2009-04-08T00:00:00Z","timestamp":1239148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gerontology"],"abstract":"<jats:p>It is well known that apolipoprotein E (apo E) has a significant role in the metabolism of plasma cholesterol through receptor-mediated endocytosis of lipoproteins containing apo B 100. We have reported that intravenous administration of apo E increases the clearance of lipoproteins containing apo B 100, resulting in reduced plasma cholesterol levels in WHHL rabbits. In the present study, we investigated the effects of exogenous apo E on lipoprotein binding to cell surface receptors. Lipoproteins were enriched in apo E by the incubation with purified apo E. Among lipoproteins enriched in apo E, VLDL and intermediate-density lipoprotein demonstrated higher binding affinity to cell surface receptor on fibroblast than respective unmodified lipoproteins. In competitive binding study, VLDL enriched by apo E (VLDL+E) completely suppressed 125I-LDL binding to human skin fibro-blasts and showed the greatest binding activity among different lipoprotein classes, whereas both VLDL and LDL incompletely suppressed a binding of 125I-VLDL+E. This result indicated that apo-E-rich VLDL can bind not only to LDL receptor but to binding sites other than LDL receptors.<\/jats:p>","DOI":"10.1159\/000213232","type":"journal-article","created":{"date-parts":[[2009,4,16]],"date-time":"2009-04-16T11:28:57Z","timestamp":1239881337000},"page":"42-48","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Exogenous Apo E on the Cellular Binding of Lipoproteins"],"prefix":"10.1159","author":[{"given":"Hiroshi","family":"Mokuno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun","family":"Ishibashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Shimano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takanari","family":"Gothoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumimaro","family":"Takaku","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,4,8]]},"container-title":["Gerontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/213232","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000213232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:01:04Z","timestamp":1777608064000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000213232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,8]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000213232","archive":["Portico"],"relation":{},"ISSN":["0304-324X","1423-0003"],"issn-type":[{"value":"0304-324X","type":"print"},{"value":"1423-0003","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,8]]}}}