{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:57:54Z","timestamp":1777946274562,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,4,17]],"date-time":"2009-04-17T00:00:00Z","timestamp":1239926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,4,17]],"date-time":"2009-04-17T00:00:00Z","timestamp":1239926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemostasis"],"abstract":"<jats:p>Conflicting reports have been published concerning the spontaneous release of prostacyclin (PGI&lt;sub&gt;2&lt;\/sub&gt;) from endothelial cells and vascular tissue in vitro. In this study the effect of using various incubation medias on the release of PGI&lt;sub&gt;2&lt;\/sub&gt; and 6-keto-PGF&lt;sub&gt;1\u03b1&lt;\/sub&gt; from primary cultures of confluent endothelial cell monolayers (ECM), grown from human umbilical cord veins, was examined. The incubations were carried out for 5 min at 22 \u00b0C on a rocker platform. The release of these compounds differed substantially with the various incubation media. Hypotonic and thrombin-containing media produced the greatest release. The 51Cr release from prelabeled ECM was also assayed. A high correlation existed between PGI&lt;sub&gt;2&lt;\/sub&gt;, 6-keto-PGF&lt;sub&gt;1\u03b1&lt;\/sub&gt; and 51Cr occurring in the various media. Since 51Cr release is considered to be a marker of cell membrane damage, the study indicates that under in vitro conditions the amount of PGI&lt;sub&gt;2&lt;\/sub&gt; released is related to the degree of cell injury.<\/jats:p>","DOI":"10.1159\/000214692","type":"journal-article","created":{"date-parts":[[2009,4,20]],"date-time":"2009-04-20T11:36:39Z","timestamp":1240227399000},"page":"345-352","source":"Crossref","is-referenced-by-count":4,"title":["Prostacyclin and\n                    <sup>51<\/sup>\n                    Cr Release in Cultured Human Endothelial Cells"],"prefix":"10.1159","author":[{"given":"Jan H.","family":"Brox","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arne","family":"Nord\u00f8y","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,4,17]]},"container-title":["Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/214692","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000214692","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T16:30:36Z","timestamp":1777739436000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000214692"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,17]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000214692","archive":["Portico"],"relation":{},"ISSN":["0301-0147","1423-0038"],"issn-type":[{"value":"0301-0147","type":"print"},{"value":"1423-0038","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,17]]}}}