{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:58:39Z","timestamp":1777946319693,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,4,28]],"date-time":"2009-04-28T00:00:00Z","timestamp":1240876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,4,28]],"date-time":"2009-04-28T00:00:00Z","timestamp":1240876800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemostasis"],"abstract":"<jats:p>Nocloprost, a 9\u03b2-chloro-16,16-dimethyl derivative of prostaglandin E&lt;sub&gt;2&lt;\/sub&gt; (PGE&lt;sub&gt;2&lt;\/sub&gt;), belongs to the gastric cytoprotective agents that are used in the therapy of gastric ulcer. Since methylated derivatives of PGE&lt;sub&gt;2&lt;\/sub&gt; are known to have proaggregatory effects the influence on platelets was studied. In platelet-rich citrated plasma, nocloprost ( &gt; 0.1 \u03bcmol\/l) caused aggregation with a biphasic course at higher concentrations. Aggregation induced by nocloprost (1 \u03bcmol\/l) corresponded to that induced by adenosine diphosphate (ADP) (5 \u03bcmol\/l). Activation of platelets by nocloprost was accompanied by formation of thromboxane A&lt;sub&gt;2&lt;\/sub&gt; and an increase in cytosolic calcium in In do 1-loaded platelets. At 0.1 \u03bcmol\/l it potentiated aggregation induced by low concentrations of ADP or adrenaline. The effect of nocloprost on platelets was blocked by iloprost, daltroban and indomethacin. PGE&lt;sub&gt;2&lt;\/sub&gt;, which was studied for comparison, at 0.1-1.0 \u03bcmol\/l inhibited aggregation induced by 1 \u03bcmol\/l nocloprost. The concentrations of nocloprost required for therapeutic use as antiulcer agent were lower by three orders of magnitude than those which induce human platelet aggregation.<\/jats:p>","DOI":"10.1159\/000216876","type":"journal-article","created":{"date-parts":[[2009,5,4]],"date-time":"2009-05-04T10:39:16Z","timestamp":1241433556000},"page":"203-211","source":"Crossref","is-referenced-by-count":0,"title":["Activation of Human Blood Platelets Induced by Nocloprost, a Stable Prostaglandin E\n                    <sub>2<\/sub>\n                    Derivative"],"prefix":"10.1159","author":[{"given":"E.","family":"Glusa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Richter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Amon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,4,28]]},"container-title":["Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/216876","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000216876","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T16:42:49Z","timestamp":1777740169000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000216876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000216876","archive":["Portico"],"relation":{},"ISSN":["0301-0147","1423-0038"],"issn-type":[{"value":"0301-0147","type":"print"},{"value":"1423-0038","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,28]]}}}