{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:54:23Z","timestamp":1789152863198,"version":"build-2803163510"},"reference-count":14,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2009]]},"abstract":"Mice that are homozygous for the recessive osteopetrosis spontaneous mutation <i>(op\/op)<\/i> develop severe osteopetrosis due to a defect in the production of macrophage colony-stimulating factor (M-CSF) and a deficiency in monocyte-derived osteoclasts. Our study describes a novel soft tissue finding in an osteopetrosis (B6C3Fe <i>a<\/i>\/<i>a<\/i>-<i>Csf1<\/i><sup>op<\/sup>\/J) mouse model. Tissues were obtained from B6C3Fe <i>a<\/i>\/<i>a<\/i>-<i>Csf1<\/i><sup>op<\/sup>\/J mice and age-matched wild-type mice, processed for hematoxylin and eosin sections, and comprehensive light microscopic tissue evaluation was performed. Mutant mice had characteristic traits of <i>op\/op<\/i> deficiency including missing incisors and domed skulls. Histologically, the bone marrow cavity was effaced by interweaving thick bony trabeculae consistent with osteopetrosis. An increase in a finely granular, basophilic interstitial extracellular matrix (ECM) was observed in the subcutaneous connective tissue of the <i>op\/op<\/i> mice when compared with controls. Histochemically, the ECM was negative with periodic acid Schiff and stained dark blue with alcian blue at a pH of 2.5, indicating that it is composed primarily of nonsulfated glycosaminoglycans (GAGs). This work suggests an increased ECM that is composed mainly of GAGs located in the subcutaneous tissue in <i>op\/op<\/i> mice. This increase in ECM may be related to altered matrix production or turnover because of changes in M-CSF production.<\/jats:p>","DOI":"10.1159\/000218336","type":"journal-article","created":{"date-parts":[[2009,7,6]],"date-time":"2009-07-06T10:41:43Z","timestamp":1246876903000},"page":"199-203","source":"Crossref","is-referenced-by-count":11,"title":["Increased Connective Tissue Extracellular Matrix in the <i>op\/op<\/i> Model of Osteopetrosis"],"prefix":"10.1159","volume":"76","author":[{"given":"Zaher A.","family":"Radi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto E.","family":"Guzman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosonald R.","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2009,6,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.87.12.4828"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1359%2Fjbmr.1997.12.11.1944"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1600-0714.2002.00087.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.173.1.269"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2F8756-3282%2892%2990365-4"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.84.17.6179"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood.V98.1.74"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMra040952"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-0714.1992.tb01032.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2F345442a0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2796.1997.00170.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.micron.2005.06.008"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002%2Fcne.901800107"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00318616"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/218336","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/218336","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T22:23:32Z","timestamp":1745447012000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000218336"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":14,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000218336","archive":["Portico"],"relation":{},"ISSN":["1015-2008","1423-0291"],"issn-type":[{"value":"1015-2008","type":"print"},{"value":"1423-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}