{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T11:59:32Z","timestamp":1762430372552,"version":"3.40.4"},"reference-count":28,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2009,5,27]],"date-time":"2009-05-27T00:00:00Z","timestamp":1243382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,5,27]],"date-time":"2009-05-27T00:00:00Z","timestamp":1243382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2010]]},"abstract":"<i>Background:<\/i> The massive pulmonary neutrophil influx in respiratory distress syndrome (RDS) in preterm infants has been ascribed to the effect of leukotriene B<sub>4<\/sub> (LTB<sub>4<\/sub>). <i>Objectives:<\/i> To investigate whether secretory phospholipase A<sub>2<\/sub> (sPLA<sub>2<\/sub>), the rate-limiting enzyme in LTB<sub>4<\/sub> production, is present in lungs of RDS infants and stimulates neutrophil migration. <i>Methods:<\/i> sPLA<sub>2<\/sub> was measured in tracheal aspirates from 15 preterm infants with RDS. The effect of aspirates on cord blood neutrophil migration was first measured, and the contribution of sPLA<sub>2<\/sub> was assessed by addition of its endogenous inhibitor Clara cell protein (CC16) or absorption of sPLA<sub>2<\/sub> from the aspirates. The role of intracellular signal transduction activation and LTB<sub>4<\/sub> formation in sPLA<sub>2<\/sub>-induced neutrophil migration was determined using purified sPLA<sub>2<\/sub>, several inhibitors of signal transduction, a LTB<sub>4<\/sub> synthesis inhibitor and a LTB<sub>4<\/sub> receptor antagonist. <i>Results:<\/i> All aspirates contained sPLA<sub>2<\/sub>, which significantly stimulated neutrophil migration. Addition of CC16 or absorption of sPLA<sub>2<\/sub> abolished the stimulatory effect. All inhibitors significantly reduced sPLA<sub>2<\/sub>-induced neutrophil migration. <i>Conclusions:<\/i> sPLA<sub>2<\/sub> is present in tracheal aspirates of preterm infants with RDS. Human recombinant sPLA<sub>2<\/sub> and pancreatic type sPLA<sub>2<\/sub> stimulate in vitro cord blood neutrophil migration via activation of intracellular signal transduction pathways, LTB<sub>4<\/sub> production and receptor binding. We speculate that sPLA<sub>2<\/sub> contributes to pulmonary neutrophil influx in RDS. Further studies are needed to determine the potential of sPLA<sub>2<\/sub> inhibition as a treatment for RDS.<\/jats:p>","DOI":"10.1159\/000220766","type":"journal-article","created":{"date-parts":[[2009,5,27]],"date-time":"2009-05-27T13:34:18Z","timestamp":1243431258000},"page":"1-9","source":"Crossref","is-referenced-by-count":8,"title":["Pulmonary Secretory Phospholipase A<sub>2<\/sub> in Infants with Respiratory Distress Syndrome Stimulates in vitro Neutrophil Migration"],"prefix":"10.1159","volume":"97","author":[{"given":"Alexandra J.J.","family":"Schrama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan G.R.","family":"Elferink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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