{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:22:23Z","timestamp":1790648543867,"version":"4.1.0"},"reference-count":21,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respiration"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Airway inflammation in chronic obstructive pulmonary disease (COPD) is predominately neutrophilic, but some subjects demonstrate eosinophilic airway inflammation. Whether these inflammatory phenotypes have differential cytokine and chemokine expression is unknown. &lt;i&gt;Objectives:&lt;\/i&gt; To assess the sputum concentrations of cytokines and chemokines and their response to oral corticosteroid therapy in COPD subjects with or without a sputum eosinophilia. &lt;i&gt;Methods:&lt;\/i&gt; Cytokine and chemokine concentrations were measured using the meso-scale device platform. To assess validity, recovery of exogenous spikes was examined. The concentrations of the validated mediators were measured in COPD sputum from subjects with or without a sputum eosinophilia. In a subgroup with a sputum eosinophilia, the response to oral prednisolone 10 mg for 1 month was examined. &lt;i&gt;Results:&lt;\/i&gt; The recovery in sputum of exogenous spiked mediators was &gt;80% in 11\/26 cytokines and chemokines. In supernatants from eosinophilic (n = 39) versus non-eosinophilic (n = 59) sputa, the geometric mean (95% CI) concentration was increased for IL-5 [9.0 (4.5\u201318) pg\/ml vs. 3.6 (2.7\u20136.3) pg\/ml, p = 0.03]. IL-5 alone was correlated with sputum eosinophil counts (r = 0.33, p = 0.001), and was attenuated following treatment with prednisolone [n = 9; mean difference 2.3 pg\/ml (0.2\u20134.3), p = 0.032]. &lt;i&gt;Conclusion:&lt;\/i&gt; We have validated the use of the meso-scale device platform for cytokine and chemokine measurements in the sputum supernatants in COPD. Sputum IL-5 was associated with a sputum eosinophilia and was attenuated following oral corticosteroid therapy. Whether this cytokine is important in the pathogenesis of COPD in a subgroup of patients warrants further investigation.<\/jats:p>","DOI":"10.1159\/000221902","type":"journal-article","created":{"date-parts":[[2009,5,27]],"date-time":"2009-05-27T13:34:42Z","timestamp":1243431282000},"page":"256-262","source":"Crossref","is-referenced-by-count":48,"title":["Sputum IL-5 Concentration Is Associated with a Sputum Eosinophilia and Attenuated by Corticosteroid Therapy in COPD"],"prefix":"10.1159","volume":"78","author":[{"given":"M.","family":"Bafadhel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Saha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Siva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"McCormick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Rugman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Dodson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.D.","family":"Pavord","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Newbold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.E.","family":"Brightling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2009,5,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.06.00025805"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200703-456SO"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-6749%2893%2990078-T"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1034%2Fj.1399-3003.2000.15.14.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.2147%2Fcopd.2006.1.1.39"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2800%2902872-5"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.00146306"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1136%2Fthorax.58.7.589"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1067%2Fmai.2000.106375"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.01.00098501"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1136%2Fthorax.57.8.667"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002%2Fstem.5530080713"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.01.00229701"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200701-085OC"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1513%2Fpats.200402-009MS"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.03.00046402"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1378%2Fchest.124.5.1909"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004080000055"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2007.02735.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.1996.d01-386.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1164%2Frccm.200506-859OC"}],"container-title":["Respiration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/221902","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/221902","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T22:51:39Z","timestamp":1745448699000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000221902"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":21,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000221902","archive":["Portico"],"relation":{},"ISSN":["0025-7931","1423-0356"],"issn-type":[{"value":"0025-7931","type":"print"},{"value":"1423-0356","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}