{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:02:20Z","timestamp":1761058940795,"version":"3.40.4"},"reference-count":8,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2009]]},"abstract":"A 55-year-old herpetologist developed rhinitis, asthma, urticaria and anaphylaxis when handling 4 different viper snake venoms. Allergen characterizations were done using SDS-PAGE, IgE immunoblotting and IgE inhibition experiments. The most prominent immunoreactive proteins were analyzed by MALDI-TOF mass spectrometry, and peptide identity was demonstrated by homology with known peptide sequences. SDS-PAGE showed several protein bands ranging from 5 to 99 kDa in each of the 4 snake venoms. Immunoblotting demonstrated 4 IgE-binding bands in the <i>Bothrops<\/i> extract of about 60, 28, 14 and 7 kDa. The bands of 28 and 14 kDa were also present in <i>Lachesis muta.<\/i> Two IgE-binding proteins of about 50 and 35 kDa were found in <i>Bothrops atrox<\/i> and <i>L. muta, <\/i>respectively. A strong inhibition of IgE binding to immobilize <i>Bothrops asper<\/i> proteins was observed after preabsorption of sera with <i>B. asper<\/i>, <i>B. atrox,<\/i><i>Bothrops xanthograma<\/i> and <i>L. muta<\/i> extracts. MALDI-TOF analysis showed a 14-kDa phospholipase and the 60- and 28-kDa proteins showed significant similarity with metalloproteinases. In this report we have characterized the snake venom allergens that can elicit IgE-mediated symptoms.<\/jats:p>","DOI":"10.1159\/000222684","type":"journal-article","created":{"date-parts":[[2009,6,8]],"date-time":"2009-06-08T14:51:42Z","timestamp":1244472702000},"page":"307-310","source":"Crossref","is-referenced-by-count":13,"title":["Characterization of Allergens in Four South American Snake Species"],"prefix":"10.1159","volume":"150","author":[{"given":"Mauro F.","family":"Madero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"G\u00e1mez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro A.","family":"Madero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar","family":"Fern\u00e1ndez-Nieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaqu\u00edn","family":"Sastre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"del Pozo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,6,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.toxicon.2007.11.022"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-3156.2000.00585.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.93.25.14440"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1136%2Fbmj.329.7475.1129"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2FS1081-1206%2810%2961140-4"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2222.2000.00697.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1006%2Fabbi.1999.1492"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.biocel.2005.10.011"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/222684","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/222684","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T23:00:03Z","timestamp":1745449203000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000222684"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":8,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000222684","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2009]]}}}