{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T03:29:41Z","timestamp":1782185381353,"version":"3.54.5"},"reference-count":24,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cerebrovasc Dis"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Leukocyte-platelet aggregates appear to be a stable and sensitive marker of platelet activation as suggested by studies in coronary heart disease. We tested the hypothesis that leukocyte-platelet aggregates are increased after ischemic stroke and investigated the contribution of different leukocyte subtypes to such increase. &lt;i&gt;Methods:&lt;\/i&gt; We serially determined granulocyte-, lymphocyte- and monocyte-platelet aggregates, using flow cytometry at days 1, 2, 3, 5, 7, 10, and 90 in patients with ischemic stroke (n = 45) and in age- and sex-matched healthy control subjects (n = 30). &lt;i&gt;Results:&lt;\/i&gt; Granulocyte-platelet aggregates (granulocytes with \u22651 platelet\/\u03bcl) were more common in patients than control subjects from day 1 through day 10 (p &lt; 0.04, respectively), but not on day 90 after stroke. The percentage of granulocytes forming aggregates was increased on days 1\u20133 after stroke but not at other time points. Lymphocyte-platelet aggregates were not more common at any time point after stroke. Total numbers and percentages of monocytes forming platelet aggregates were significantly increased on day 2 (p = 0.003), but not at other time points after stroke. &lt;i&gt;Conclusion:&lt;\/i&gt; The 3 leukocyte subtypes showed different kinetics regarding aggregate formation with platelets after ischemic stroke. Increase of monocyte-platelet aggregates is short-lived and may reflect an acute reaction to cerebral ischemia, whereas granulocyte-platelet aggregate formation persists into the subacute phase, suggesting that they are a particularly sensitive parameter reflecting both prothrombotic and inflammatory processes after stroke.<\/jats:p>","DOI":"10.1159\/000228710","type":"journal-article","created":{"date-parts":[[2009,7,15]],"date-time":"2009-07-15T13:29:55Z","timestamp":1247664595000},"page":"276-282","source":"Crossref","is-referenced-by-count":55,"title":["Leukocyte-Platelet Aggregates in Acute and Subacute Ischemic Stroke"],"prefix":"10.1159","volume":"28","author":[{"given":"L.","family":"Marquardt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Anders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Buggle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Palm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Hellstern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.J.","family":"Grau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2009,7,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038%2Fnm1102-1227"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.20012044"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1161%2F01.RES.0000252802.25497.b7"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.blre.2006.06.001"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1161%2FATVBAHA.107.158915"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1080%2F09537100802105040"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1161%2F01.STR.0000034398.34938.20"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1161%2F01.STR.0000074032.64049.47"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.93.21.11877"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1161%2Fhc3801.095588"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0735-1097%2801%2901485-1"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0735-1097%2897%2900510-X"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1161%2F01.CIR.0000015700.27754.6F"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1161%2F01.STR.0000236638.75591.61"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0092-8674%2893%2990327-M"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.128.4.661"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1080%2F095371000403080"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.91.19.8767"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038%2F359848a0"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002%2Fart.1780350824"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038%2F35393"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1006%2Fexnr.2001.7835"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-510X%2801%2900590-1"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.2004.04983.x"}],"container-title":["Cerebrovascular Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/228710","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/228710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T21:44:07Z","timestamp":1745444647000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000228710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":24,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000228710","archive":["Portico"],"relation":{},"ISSN":["1015-9770","1421-9786"],"issn-type":[{"value":"1015-9770","type":"print"},{"value":"1421-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}