{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T07:48:28Z","timestamp":1747381708446,"version":"3.40.4"},"reference-count":20,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Objective:&lt;\/i&gt; IGF-I stimulates multiple functions of connective tissue cells and its activity is modulated by IGF-binding proteins (BPs). Some metalloproteinases are expected to modify IGF-I activity by digestion of IGF-BPs. It was decided to evaluate the concentration of IGF-I, IGF-BPs and the activity of gelatinases A and B in knee exudates of children with post-traumatic damage (PTD) and children with juvenile idiopathic arthritis (JIA) in comparison with those in the sera of the same patients. &lt;i&gt;Methods:&lt;\/i&gt; ELISA (for IGF-I assay), polyacrylamine gel electrophoresis following Western immunoblotting (for IGF-I and IGF-BPs expression), and zymography (for gelatinase detection) were used. &lt;i&gt;Results:&lt;\/i&gt; The knee exudates, especially those taken from patients with JIA, contained large amounts of IGF-I. The exudates of PTD and JIA patients contained some forms of IGF-BP-1 of molecular weight lower than those occurring in serum. Low expression BP-3 and high activity of gelatinase B were detected in the JIA exudates. &lt;i&gt;Conclusions:&lt;\/i&gt; The high gelatinase activities in exudates imply joint tissue damage. The cellular response to damage of this kind is an increase in IGF-I production, which stimulates repair processes. High proteolytic activities of gelatinase B in JIA patients may lower the amount of BP-3, possibly causing a relative decrease of IGF-I concentration and impairing the reparation processes stimulated by IGF-I.<\/jats:p>","DOI":"10.1159\/000228902","type":"journal-article","created":{"date-parts":[[2009,10,3]],"date-time":"2009-10-03T04:00:03Z","timestamp":1254542403000},"page":"260-266","source":"Crossref","is-referenced-by-count":10,"title":["IGF-I and IGF-Binding Proteins in Articular Exudates of Children with Post-Traumatic Knee Damage and Juvenile Idiopathic Arthritis"],"prefix":"10.1159","volume":"76","author":[{"given":"Tomasz","family":"Guszczyn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Rzeczycka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janusz","family":"Popko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,10,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01972702"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.berh.2005.01.004"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097%2F01.blo.0000143805.64755.4f"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.joca.2005.10.011"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02331388"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01953688"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1556%2FAPhysiol.92.2005.2.1"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1385%2FENDO%3A12%3A2%3A121"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1749-6632.1993.tb26201.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2F227680a0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.103.3.1021"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.81.1.150"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.86.12.5870"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.142.11.4818"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ghir.2008.01.006"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1677%2Fjoe.0.1490397"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2F0955-2235%2895%2900017-8"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2F1357-2725%2895%2900121-2"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.274.11.6935"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00464-006-9103-x"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/228902","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/228902","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T21:46:03Z","timestamp":1745444763000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000228902"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":20,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2009,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000228902","archive":["Portico"],"relation":{},"ISSN":["1015-2008","1423-0291"],"issn-type":[{"type":"print","value":"1015-2008"},{"type":"electronic","value":"1423-0291"}],"subject":[],"published":{"date-parts":[[2009]]}}}