{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:01:51Z","timestamp":1777899711041,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,7,23]],"date-time":"2009-07-23T00:00:00Z","timestamp":1248307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,7,23]],"date-time":"2009-07-23T00:00:00Z","timestamp":1248307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Appl Imm"],"abstract":"<jats:p>The influence of type and degree of substitution of B 512 dextran fractions on reactivity with rabbit antibodies against unmodified B 512 dextran was studied in heterologous passive cutaneous anaphylaxis in guinea pigs. Low degrees of substitution, i.e. 1 substituent group per 7\u201330 glucose residues, affected reactivity with anti-dextran only slightly or not at all. This was shown for the following substituents: sulphate, carboxymethyl, phosphate, diethylaminoethyl, hydroxypropyl, butyryl, caprylyl, stearoyl, and fluoresceinoyl groups. With high degress of substitution, i.e. 1\u20133 substituents per 2 glucose residues, reactivity with anti-B-512-dextran is completely abolished for some substituents, and a new immunological identity conferred on the substituted dextran. Strongly charged groups like sulphate, carboxymethyl and diethylaminoethyl abolish reactivity with anti-B-512-dextran at relatively lower degrees of substitution than more neutral groups like methyl and acetyl. The anti-B-512-dextran represents a specific reagent for \u03b1-1,6-linked polyglucose, as evidenced by complete cross-reactivity with synthetic linear dextran; its specificity is emphasized by non-reactivity with \u03b1-1,6-linked synthetic mannan, the monomeric residues of the two polymers differing only in position of the C&lt;sub&gt;2&lt;\/sub&gt; hydroxyl groups.<\/jats:p>","DOI":"10.1159\/000231338","type":"journal-article","created":{"date-parts":[[2009,7,28]],"date-time":"2009-07-28T02:24:43Z","timestamp":1248747883000},"page":"505-512","source":"Crossref","is-referenced-by-count":9,"title":["Effect of Substitution on Reactivity of B 512 Dextran Fractions with Anti-B 512 Dextran in Heterologous Passive Cutaneous Anaphylaxis"],"prefix":"10.1159","author":[{"given":"Wolfgang","family":"Richter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,7,23]]},"container-title":["International Archives of Allergy and Applied Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/231338","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000231338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:12:18Z","timestamp":1777630338000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000231338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,23]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000231338","archive":["Portico"],"relation":{},"ISSN":["0020-5915","2571-6662"],"issn-type":[{"value":"0020-5915","type":"print"},{"value":"2571-6662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7,23]]}}}