{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:23:38Z","timestamp":1777901018783,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,8,5]],"date-time":"2009-08-05T00:00:00Z","timestamp":1249430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,8,5]],"date-time":"2009-08-05T00:00:00Z","timestamp":1249430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Appl Imm"],"abstract":"<jats:p>We show here an automated (50 samples\/h) assay for serum IgG4 having a throughput time of 40 min per sample and a sensitivity of 10 \u03bcg\/ml. The assay procedure is based on the inhibition by sample of the agglutination reaction between monoclonal anti-IgG4 antibodies and latex particles to which IgG4 myeloma protein has been coupled. Assay reliability was ascertained by testing for linearity, analytical recovery (96.4%), interassay precision (\u22648%), specificity and correlation between the results obtained with monoclonal and polyclonal anti-IgG4 antibodies (n = 84; r&lt;sub&gt;s&lt;\/sub&gt; = 0.97). Application of the assay to sera from various groups of patients indicated significantly (p &lt; 0.00005) higher geometrical means (Gx) in patients suffering from atopy (n = 87; Gx = 617 \u03bcg\/ml), atopic dermatitis (n = 28; Gx = 1,043 \u03bcg\/ml), filariasis with Onchocerca volvulus (n = 48; Gx = 1,681 \u03bcg\/ml) and Brugia malayi(n = 20; Gx = 1,078 \u03bcg\/ml) as compared to nonatopic subjects (n = 103; Gx = 302 \u03bcg\/ml) and randomized paired maternal\/cord sera (n = 41; Gx = 276 and 296 \u03bcg\/ml, respectively). IgG4 in the paired maternal\/cord sera correlated (r = 0.98; p &lt;0.00005). There was no significant influence of age or sex on the IgG4 levels either among the nonatopics or the atopies even though low IgG4 (\u226430 \u03bcg\/ml) was more common among women. The results suggest that IgG4 and IgE responses are somehow closely related in atopic and parasite-infested patients at the physiological, pathogenic or genetic level.<\/jats:p>","DOI":"10.1159\/000234141","type":"journal-article","created":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T09:46:18Z","timestamp":1249638378000},"page":"238-244","source":"Crossref","is-referenced-by-count":9,"title":["Raised Serum IgG4 Levels in Patients with Atopy and Filariasis: Application of an Automated Particle-Counting Immunoassay Using Monoclonal Antibody"],"prefix":"10.1159","author":[{"given":"C.G.M.","family":"Magnusson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.Y.","family":"Cesbron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Djurup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Capron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.G.O.","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,8,5]]},"container-title":["International Archives of Allergy and Applied Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/234141","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000234141","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:49:08Z","timestamp":1777632548000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000234141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000234141","archive":["Portico"],"relation":{},"ISSN":["0020-5915","2571-6662"],"issn-type":[{"value":"0020-5915","type":"print"},{"value":"2571-6662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,5]]}}}