{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:26:13Z","timestamp":1777901173926,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,8,5]],"date-time":"2009-08-05T00:00:00Z","timestamp":1249430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,8,5]],"date-time":"2009-08-05T00:00:00Z","timestamp":1249430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Appl Imm"],"abstract":"<jats:p>In this study we investigated the modulation of natural killer (NK) cell activity by various histamine receptor antagonists in vitro. The histamine H&lt;sub&gt;2&lt;\/sub&gt;-receptor antagonists cimetidine, ranitidine and tiotidine suppressed NK cell cytotoxicity (NKCC) at a high concentration (10\u2013\u20133M). Cimetidine enhanced NKCC of Ficoll-Hypaque-separated lymphocytes and of lymphocytes enriched for NKCC by Percoll density gradient centrifugation. The enhancing effect of cimetidine was dose-dependent at final concentrations of 10\u2013\u20134\u2013\u201310\u2013\u20137M and did not require the presence of adherent cells\/monocytes. Ranitidine did not affect NKCC over a wide range of concentrations. Tiotidine strongly enhanced NKCC of low-density, large granular lymphocyte-enriched mononuclear cells (MNC) in the presence of adherent cells\/monocytes, but was ineffective in nonadherent effector cells. All H&lt;sub&gt;2&lt;\/sub&gt;-receptor antagonists clearly antagonized histamine-induced NKCC enhancement in monocyte-containing effector cells. Clemastin, a specific H&lt;sub&gt;1&lt;\/sub&gt;-receptor antagonist, effectively suppressed NKCC. This effect was mimicked by a clemastin isomer with very low affinity for H&lt;sub&gt;1 &lt;\/sub&gt;receptors. We conclude that (1) cimetidine enhances NKCC in vitro by a mechanism of action that is not specifically related to antagonism of H&lt;sub&gt;2&lt;\/sub&gt;-receptors, (2) tiotidine displays mixed agonist\/antagonist properties for MNC H&lt;sub&gt;2&lt;\/sub&gt;-receptors and (3) NK-suppressive properties of clemastin are unrelated to H&lt;sub&gt;1&lt;\/sub&gt;-receptor antagonism.<\/jats:p>","DOI":"10.1159\/000234431","type":"journal-article","created":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T09:46:18Z","timestamp":1249638378000},"page":"247-255","source":"Crossref","is-referenced-by-count":11,"title":["Differential Effects of Histamine Receptor Antagonists on Human Natural Killer Cell Activity"],"prefix":"10.1159","author":[{"given":"Kristoffer","family":"Hellstrand","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svante","family":"Hermodsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,8,5]]},"container-title":["International Archives of Allergy and Applied Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/234431","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000234431","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:53:50Z","timestamp":1777632830000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000234431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000234431","archive":["Portico"],"relation":{},"ISSN":["0020-5915","2571-6662"],"issn-type":[{"value":"0020-5915","type":"print"},{"value":"2571-6662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,5]]}}}