{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:34:24Z","timestamp":1777901664025,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T00:00:00Z","timestamp":1249603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T00:00:00Z","timestamp":1249603200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Appl Imm"],"abstract":"<jats:p>The effects of mucosal T-cell activation on human small intestinal glycoprotein biosynthesis have been examined during short-term culture in vitro. The incorporation of 3H-glucosamine into tissue and secreted glycoproteins was determined. Activation of mucosal T lymphocytes of normal duodenal biopsies with the monoclonal anti-CD3 antibody significantly increased both the total glucosamine incorporation into glycoproteins (p &lt; 0.01) and the secretion of in vitro radiolabelled glycoproteins (p &lt; 0.001). This effect was inhibited by ciclosporin. Secretion of glycoproteins was also stimulated by culture with pokeweed mitogen. In patients with coeliac disease, culture of small intestinal biopsies with anti-CD3 antibody significantly increased (p &lt; 0.05) glycoprotein biosynthesis in treated patients, but had no stimulatory effect in untreated patients with villous atrophy. These results show that activation of mucosal T lymphocytes induces quantitative changes in intestinal glycoprotein synthesis and secretion and T lymphocytes therefore have an important role in non-specific intestinal defences. The results are consistent with the suggestion that increased glycoprotein synthesis and secretion in untreated coeliac mucosa result from T-cell activation.<\/jats:p>","DOI":"10.1159\/000235276","type":"journal-article","created":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T09:46:18Z","timestamp":1249638378000},"page":"35-40","source":"Crossref","is-referenced-by-count":5,"title":["T Lymphocyte Stimulation of Human Small Intestinal Glycoprotein Biosynthesis: Effects of Anti-CD3 Antibody on Normal and Coeliac Mucosa"],"prefix":"10.1159","author":[{"given":"J.E.","family":"Crabtree","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.V.","family":"Heatley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.K.","family":"Trejdosiewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.S.","family":"Losowsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,8,7]]},"container-title":["International Archives of Allergy and Applied Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/235276","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000235276","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:07:23Z","timestamp":1777633643000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000235276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,7]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000235276","archive":["Portico"],"relation":{},"ISSN":["0020-5915","2571-6662"],"issn-type":[{"value":"0020-5915","type":"print"},{"value":"2571-6662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,7]]}}}