{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T22:27:52Z","timestamp":1778797672302,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T00:00:00Z","timestamp":1249603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T00:00:00Z","timestamp":1249603200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Appl Imm"],"abstract":"<jats:p>Human umbilical vascular endothelial cells (HUVEC) stimulated with recombinant human IFN-\u03b3 were investigated for expression of HLA class II molecules, toxic shock syndrome toxin-1 (TSST-l)-binding activity and accessory cell (AC) activity in TSST-1-induced T cell activation. HUVEC stimulated with recombinant human IFN-\u03b3 ranging from 10 to 1,000 units\/ml for 72 h express HLA class II molecules. Intensity of the expression was dependent on the concentration of IFN-\u03b3. HUVEC stimulated with 1,000 units\/ml of IFN-\u03b3 for 72 h exhibited 125I-TSST-l-binding that was blocked by the unlabeled toxin and monoclonal antibodies (mAb) to DR\/DP. The activity was not removed by paraformaldehyde fixation. The IFN-\u03b3-stimulated HUVEC exhibited AC activity in TSST-1-induced IL-2 production by T cells from human peripheral blood mononuclear cells. The activity was blocked by mAb to DR. The above two activities were also observed in L cells transfected with DR2 genes but not in the unstimulated HUVEC and control L cells. In view of the fact that TSST-1 binds directly to HLA class II molecules and activates human T cells in association with HLA class II molecules on AC, it is likely that the acquisition of TSST-1-binding activity and AC activity in the toxin-induced T cell activation is mediated by the expression of HLA class II molecules. Vascular endothelial cells may play an important role in the development of pathological changes in TSS patients.<\/jats:p>","DOI":"10.1159\/000235535","type":"journal-article","created":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T09:46:18Z","timestamp":1249638378000},"page":"55-61","source":"Crossref","is-referenced-by-count":8,"title":["Activation of Human Vascular Endothelial Cells by IFN-\u03b3: Acquisition of HLA Class II Expression, TSST-1-Binding Activity and Accessory Activity in T Cell Activation by the Toxin"],"prefix":"10.1159","author":[{"given":"Minako","family":"Araake","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiko","family":"Uchiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken\u2019ichi","family":"Imanishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Jie","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,8,7]]},"container-title":["International Archives of Allergy and Applied Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/235535","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000235535","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:11:52Z","timestamp":1777633912000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000235535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,7]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000235535","archive":["Portico"],"relation":{},"ISSN":["0020-5915","2571-6662"],"issn-type":[{"value":"0020-5915","type":"print"},{"value":"2571-6662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,7]]}}}