{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:38:27Z","timestamp":1777901907199,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,9,2]],"date-time":"2009-09-02T00:00:00Z","timestamp":1251849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,9,2]],"date-time":"2009-09-02T00:00:00Z","timestamp":1251849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"abstract":"<jats:p>Fourteen Brown-Norway rats were pretreated with physiological saline (n = 7) or 500 \u03bcg Sephadex (n = 7) intratracheally. 24 h later, a bronchial provocation test was performed under pentobarbital anaesthesia using increasing doses of acetylcholine aerosol and the degree of bronchospasm was measured using a modified Konzett-R\u00f6ssler method. Subsequently, leucocyte counts were determined in the bronchoalveolar lavage fluid (BALF), BALF cells were differentiated, and the chemiluminescence of the BALF leucocytes were measured. Finally, the lungs were removed and histologically examined. The cell count in the BALF was significantly (p &lt; 0.05) increased in the animals pretreated with Sephadex compared to those in the saline group (mean value \u00b1 SEM: 0.38 \u00b1 0.07 vs. 0.15 \u00b1 0.02 \u00d7 106\/ml). This difference was also reflected in the chemiluminescence measurements (2.51 \u00b1 0.53 vs. 0.20 \u00b1 0.03 \u00d7 106 counts\/0.5 ml). In the Sephadex-treated animals there was also a significant increase in the absolute number of neutrophil (0.040 \u00b1 0.010 vs. 0.011 \u00b1 0.002 \u00d7 106\/ml) and, in particular, eosinophil granulocytes (0.188 \u00b1 0.055 vs. 0.003 \u00b1 0.001 \u00d7 106\/ml) in the total leucocytes of the BALF. Lung histology showed massive perialveolar and peribronchial oedema and granulomatous infiltrates, primarily with eosinophils, after intratracheal application of Sephadex; these findings were not observed in the saline group. None of these changes in the rats pretreated with Sephadex manifested themselves in increased bronchial reactivity to acetylcholine aerosol. It is uncertain if the Sephadex-induced increase in the eosinophil count is accompanied by an activation of this cell population, which appears to be of importance for the occurrence of bronchial hyperreactivity. It is clear from this animal model that the presence of inflammatory cells in the BALF and lung tissue of rats does not in itself result in increased reactivity of the airways.<\/jats:p>","DOI":"10.1159\/000236196","type":"journal-article","created":{"date-parts":[[2009,9,15]],"date-time":"2009-09-15T08:17:46Z","timestamp":1253002666000},"page":"266-272","source":"Crossref","is-referenced-by-count":21,"title":["Intratracheal Application of Sephadex in Rats Leads to Massive Pulmonary Eosinophilia without Bronchial Hyperreactivity to Acetylcholine"],"prefix":"10.1159","author":[{"given":"Rolf","family":"Kubin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Deschl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Linssen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Otto-Henning","family":"Wilhelms","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,9,2]]},"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/236196","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000236196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:13:40Z","timestamp":1777634020000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000236196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,2]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000236196","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,2]]}}}