{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:42:40Z","timestamp":1777902160594,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T00:00:00Z","timestamp":1251936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T00:00:00Z","timestamp":1251936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"abstract":"<jats:p>Airborne endotoxins play a role in a variety of occupational diseases such as byssinosis and humidifier\u2019s disease and are associated with pulmonary and systemic symptoms. An excess generation of oxygen free radicals (including superoxide anion, O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt;) by inflammatory cells has been suggested in endotoxemia. We have studied the release of superoxide from guinea-pig lung lavage and interstitial cells and blood monocytes (BMs) at different times after an acute inhalation exposure to bacterial endotoxin. O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; generation was measured by the O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; dismutase-inhibitable reduction of ferricytochrome c, after stimulation with phorbol-myristate-acetate (PMA) or opsonized zymosan (OZ). After endotoxin exposure the spontaneous release of O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; remained unchanged for the three cell types. From 4 h after exposure until 48 h afterwards, lung lavage cells produced more O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; after PMA or OZ stimulation than did cells from unexposed guinea pigs. The pattern of O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; generation by interstitial cells followed that of lung lavage macrophages. O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; production remained unchanged in BMs at all times. These results suggest that endotoxin inhalation induced a priming of lavage and interstitial cells, mainly macrophages, associated with an increase in O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; production. As O\u2013\u2013&lt;sub&gt;2&lt;\/sub&gt; production by BMs remained unchanged, this cell is unlikely to be responsible for the systemic symptoms seen after endotoxin inhalation.<\/jats:p>","DOI":"10.1159\/000236707","type":"journal-article","created":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T09:04:16Z","timestamp":1252055056000},"page":"42-47","source":"Crossref","is-referenced-by-count":5,"title":["Oxygen Metabolites from Lavage and Interstitial Lung Cells after Inhalation of Endotoxin in Guinea Pigs"],"prefix":"10.1159","author":[{"given":"Vincent","family":"Hsieh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Amoruso-Marchat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ragnar","family":"Rylander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara S.","family":"Polla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,9,3]]},"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/236707","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000236707","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:21:43Z","timestamp":1777634503000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000236707"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,3]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000236707","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,3]]}}}