{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:44:23Z","timestamp":1777902263709,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T00:00:00Z","timestamp":1252022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T00:00:00Z","timestamp":1252022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"abstract":"<jats:p>Antigen-stimulated contraction and release of chemical mediators were examined in saline- or Sephadex-treated rat lung parenchymal strips. Sephadex treatment caused eosinophilia in the blood and the lung tissue. Antigen challenge of the isolated parenchymal strips in Sephadex-treated rat was followed by passive sensitization, resulted in an augmented contraction and elevated releases of thromboxane (TX) B&lt;sub&gt;2&lt;\/sub&gt; and peptide-leukotrienes (p-LTs) in bath fluid compared with those of saline-treated control. Although 5-hydroxytryptamine (5-HT) and histamine were significantly released after antigen challenge, the levels were not different between saline- and Sephadex-treated groups. DP-1904, a selective thromboxane synthetase inhibitor, and methysergide but not atropine significantly reduced the augmented contraction and inhibited the elevated TXB&lt;sub&gt;2&lt;\/sub&gt; release in the Sephadex-treated group. Similar increased contraction and the elevated TXB&lt;sub&gt;2&lt;\/sub&gt; release above were observed when Sephadex-treated rat lung strips were stimulated by exogenous 5-HT and LTD&lt;sub&gt;4&lt;\/sub&gt;. These augmented contractions were closely correlated with the increase in TXB&lt;sub&gt;2&lt;\/sub&gt; level (r = 0.83; p &lt; 0.01). In addition, contraction to U-46619, a thromboxane mimetic, was significantly greater in Sephadex-treated rat lung strips. Our results indicate that the ability of Sephadex-treated rat lung tissue to synthesize newly generated mediators such as TXA&lt;sub&gt;2&lt;\/sub&gt; and p-LTs is increased, and the spasmogenic susceptibility of the lung tissue to TXA&lt;sub&gt;2&lt;\/sub&gt; itself is modified by Sephadex treatment, suggesting these are due to the augmented contraction in an established hyperresponsiveness state induced by Sephadex.<\/jats:p>","DOI":"10.1159\/000236873","type":"journal-article","created":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T09:04:16Z","timestamp":1252055056000},"page":"401-409","source":"Crossref","is-referenced-by-count":7,"title":["Possible Role of Thromboxane A\n                    <sub>2<\/sub>\n                    in Hyperresponsiveness of Isolated Rat Lung Tissue in a Sephadex-Induced Eosinophilia Model"],"prefix":"10.1159","author":[{"given":"Mitsutaka","family":"Takami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyomi","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimi","family":"Takata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhisa","family":"Furuhama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Tsukada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,9,4]]},"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/236873","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000236873","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:24:32Z","timestamp":1777634672000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000236873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,4]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000236873","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,4]]}}}