{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:09:03Z","timestamp":1777907343936,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T00:00:00Z","timestamp":1252022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T00:00:00Z","timestamp":1252022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"abstract":"<jats:p>Allergic disease presents an increasing health problem in industrialised countries. Despite improved diagnosis and drug therapy, the number of fatalities from asthma and anaphylaxis continues to rise. A key element in the aetiology of the allergic response to inert protein antigens is an inherent imbalance in the atopic allergen specific CD4+ T cells, resulting in a dominance of the pathological, IL-4 producing, T helper 2 phenotype which favours B cell IgE production. Previous work from our laboratory, and elsewhere, has established that CD8+ T cells play an important role in regulating the magnitude and duration of IgE responses and may be vital for the restoration of IgE immunologic homoeostasis in \u2018normal\u2019 non-atopic humans and low\/medium responder rodents. We have found that CD8+ T cell depletion during a critical period after immunization produces a high and persistent IgE response in otherwise IgE hyporesponsive animals. We and others have demonstrated that small, but important, quantities of soluble antigen can enter the MHC class I pathway and, using ovalbumin (OVA)-transfected antigen-presenting cells, have succeeded in cloning OVA-specific, MHC class I-restricted rat \u03b1\/\u03b2 T cell receptor (TcR) positive CD8+ T cells. These cells universally secrete IFN-\u03b3 (5\u201369 ng\/ml) and IL-2 (7.6\u201337.0 U\/ml) and occasionally IL-4 (16.0\u20138\/1.0 IU). When adoptively transferred, 1 \u00d7 106 of these cloned cells inhibit IgE production in vivo by as much as 50-fold and this effect can be titrated down to 1 \u00d7 103 cells. The IgE regulatory activity of the clones appears unrelated to their cytolytic potential or to their capacity to make IFN-\u03b3.<\/jats:p>","DOI":"10.1159\/000237572","type":"journal-article","created":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T09:04:16Z","timestamp":1252055056000},"page":"279-280","source":"Crossref","is-referenced-by-count":2,"title":["Generation of Rat MHC Class-I-Restricted Ovalbumin-Specific IgE Inhibitory CD8+ T Cell Clones"],"prefix":"10.1159","author":[{"given":"P.A.","family":"MacAry","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.J.","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.M.","family":"Kemeny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,9,4]]},"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/237572","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000237572","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:33:00Z","timestamp":1777642380000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000237572"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,4]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000237572","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,4]]}}}