{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:07:47Z","timestamp":1777907267528,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T00:00:00Z","timestamp":1252022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T00:00:00Z","timestamp":1252022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"abstract":"<jats:p>Human mast cells (MC) were derived from umbilical cord blood and bone marrow progenitors cultured in the presence of a conditioned medium from a human mastocytosis cell strain and recombinant human kit ligand (rhKL). KL induced MC of predominantly two immunophenotypes, MC&lt;sub&gt;T&lt;\/sub&gt; and MC&lt;sub&gt;TC&lt;\/sub&gt;. In contrast, the conditioned medium induced MC subtypes MC&lt;sub&gt;TC&lt;\/sub&gt; and a third subtype, MC&lt;sub&gt;C&lt;\/sub&gt;, positive for chymase but negative for tryptase. This study clearly demonstrates that a third type of MC, MC&lt;sub&gt;C&lt;\/sub&gt;, can be induced in vitro from normal human progenitors.<\/jats:p>","DOI":"10.1159\/000237576","type":"journal-article","created":{"date-parts":[[2009,9,4]],"date-time":"2009-09-04T09:04:16Z","timestamp":1252055056000},"page":"289-290","source":"Crossref","is-referenced-by-count":2,"title":["Conditioned Media Obtained from a Human Mastocytosis Cell Strain Induce Mast Cells Expressing Chymase but not Tryptase from Human Progenitors"],"prefix":"10.1159","author":[{"given":"Lixin","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven A.","family":"Krilis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,9,4]]},"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/237576","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000237576","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:33:05Z","timestamp":1777642385000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000237576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,4]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000237576","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,4]]}}}