{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T03:21:48Z","timestamp":1770434508417,"version":"3.49.0"},"reference-count":14,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt;Defective nitric oxide (NO)-mediated vasodilation is widely regarded as an underlying cause of hypertension in pre-eclampsia, although there are also arguments against this hypothesis. &lt;i&gt;Methods:&lt;\/i&gt;We examined both the mRNA levels and the presence of a Glu298Asp substitution in the NO synthase (NOS) gene, as well as the NO metabolite concentration, in placentas and maternal sera from women with pre-eclampsia and in normotensive pregnant controls (25\u201340 vs. 24\u201341 weeks of gestation). &lt;i&gt;Results:&lt;\/i&gt;Pre-eclamptic and control placentas did not show any significant differences in their NO metabolite levels or their NOS expression levels as measured by quantitative RT-PCR. In addition, we did not find any association between pre-eclampsia and the occurrence of the Glu298Asp amino acid substitution in the NOS gene. In contrast, high maternal circulating NO metabolites were evident in severe pre-eclampsia (p &lt; 0.0001). Although a positive correlation between circulating NO metabolites and blood pressure was not observed, uterine artery resistance measured by ultrasound was found to positively correlate with the maternal NO levels. &lt;i&gt;Conclusions:&lt;\/i&gt;Our current data suggest that an altered placental NOS pathway is unlikely to be the primary cause of pre-eclampsia and that the activation of this pathway is possibly in response to maternal symptoms.<\/jats:p>","DOI":"10.1159\/000238381","type":"journal-article","created":{"date-parts":[[2009,9,16]],"date-time":"2009-09-16T16:41:14Z","timestamp":1253119274000},"page":"239-247","source":"Crossref","is-referenced-by-count":19,"title":["Analysis of Nitric Oxide Metabolism as a Placental or Maternal Factor Underlying the Etiology of Pre-Eclampsia"],"prefix":"10.1159","volume":"68","author":[{"given":"Haruki","family":"Nishizawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanako","family":"Pryor-Koishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Machiko","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takema","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Sekiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin","family":"Tada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Kurahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Udagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,9,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9378%2896%2970341-1"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0029-7844%2899%2900007-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0029-7844%2800%2900939-X"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3109%2F10641959509015680"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097%2F00003081-200209000-00014"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS1071-5576%2800%2900060-5"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2265.2002.01626.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa031884"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.placenta.2006.05.010"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2Flabinvest.3700746"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.placenta.2005.04.011"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2F0143-4004%2895%2990013-6"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2F0002-9378%2895%2990324-0"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000292266"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/238381","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/238381","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T21:54:09Z","timestamp":1745445249000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000238381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":14,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000238381","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"value":"0378-7346","type":"print"},{"value":"1423-002X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}