{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:44:29Z","timestamp":1745469869472,"version":"3.40.4"},"reference-count":11,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2009,10,14]],"date-time":"2009-10-14T00:00:00Z","timestamp":1255478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,10,14]],"date-time":"2009-10-14T00:00:00Z","timestamp":1255478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>&lt;i&gt;Purpose:&lt;\/i&gt; Gibco\u2019s Minimum Essential Medium with Earle\u2019s salts and HEPES supplemented with glutamine, antibiotics (EB MEM) and 2% foetal calf serum (FCS) is used in European eye banks to store corneas. Although FCS is important to endothelial cell survival in this medium, it is a potential biohazard. Megacell MEM, formulated to reduce the FCS requirement of cells by a factor of 5, has therefore been evaluated as a corneal storage medium. &lt;i&gt;Methods:&lt;\/i&gt; Corneal stromal and epithelial cells were incubated in Megacell MEM (serum-free or 2% FCS) to assess their viability in these media. Endothelial cell densities of paired corneas held in either EB MEM 2% FCS or Megacell MEM (serum-free or 2% FCS) were measured over 5 weeks. Discs subsequently punched from the centre of these corneas were weighed, dried and reweighed to determine hydration levels. &lt;i&gt;Results:&lt;\/i&gt; Both corneal stromal and epithelial cells proliferated in Megacell MEM 2% FCS. Relative to EB MEM, 2% FCS Megacell MEM prolonged the viability of corneal endothelial cells and improved their morphological appearance, irrespective of whether it contained FCS or not. This was independent of corneal swelling. &lt;i&gt;Conclusion:&lt;\/i&gt; Serum-free Megacell MEM is a better storage medium than EB MEM 2% FCS for corneas destined for transplantation<\/jats:p>","DOI":"10.1159\/000246574","type":"journal-article","created":{"date-parts":[[2009,10,23]],"date-time":"2009-10-23T14:20:24Z","timestamp":1256307624000},"page":"18-25","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Megacell\u2122 MEM as a Storage Medium for Corneas Destined for Transplantation"],"prefix":"10.1159","volume":"43","author":[{"given":"Valerie A.","family":"Smith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terrell","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,10,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00154722"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1755-3768.1987.tb08508.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097%2F00003226-199509000-00004"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1136%2Fbjo.85.12.1416"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1136%2Fbjo.85.9.1075"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1167%2Fiovs.02-0601"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1167%2Fiovs.04-1078"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0161-6420%2898%2991030-2"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1755-3768.1979.tb00491.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1097%2F00003226-198404000-00002"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1097%2F00003226-199509000-00010"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/246574","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/246574","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T21:47:55Z","timestamp":1745444875000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000246574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,14]]},"references-count":11,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2009,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000246574","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"type":"print","value":"0030-3747"},{"type":"electronic","value":"1423-0259"}],"subject":[],"published":{"date-parts":[[2009,10,14]]}}}