{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T03:54:57Z","timestamp":1777866897569,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T00:00:00Z","timestamp":1255046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T00:00:00Z","timestamp":1255046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatologica"],"abstract":"<jats:p>The degree of complement activation produced by hydrogen peroxide was estimated by the inhibition of serum homolytic activity (%IHA). Sera from patients with systemic lupus erythematosus and psoriasis vulgaris were resistant to hydrogen-peroxide-mediated complement activation. %IHA negatively correlated with ceruloplasmin level or catalase activity in systemic lupus erythematosus sera, but did not correlate with transferrin level. The addition of free metal ions, FeCl&lt;sub&gt;2&lt;\/sub&gt; or CuCl&lt;sub&gt;2&lt;\/sub&gt;, promoted hydrogen-peroxide-mediated complement activation. These results suggest that hydroxyl radical is involved in complement activation and that the factors responsible for the insensitivity of pathological sera to H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt; are catalase and ceruloplasmin in the sera. Human skin fibroblasts generate superoxide and tumor necrosis factor enhanced it, but interleukin-1&lt;sub&gt;\u03b2&lt;\/sub&gt; inhibited it. Normal serum cultured with fibroblasts for 24 h showed complement activation via catalase-inhibitable process, suggesting that hydrogen peroxide has an important role in fibroblast-mediated complement activation. It is speculated that fibroblasts and complement activation by oxygen radicals have an important role in inflammation and subsequent tissue damage at the site of skin lesion.<\/jats:p>","DOI":"10.1159\/000248459","type":"journal-article","created":{"date-parts":[[2009,10,14]],"date-time":"2009-10-14T13:46:30Z","timestamp":1255527990000},"page":"107-112","source":"Crossref","is-referenced-by-count":15,"title":["Possible Role of H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    -Mediated Complement Activation and Cytokines-Mediated Fibroblasts Superoxide Generation on Skin Inflammation"],"prefix":"10.1159","author":[{"given":"M.","family":"Shingu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Nonaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nobunaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Ahamadzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,10,9]]},"container-title":["Dermatologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/248459","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000248459","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:33:21Z","timestamp":1777541601000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000248459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,9]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000248459","archive":["Portico"],"relation":{},"ISSN":["0011-9075","2571-7197"],"issn-type":[{"value":"0011-9075","type":"print"},{"value":"2571-7197","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10,9]]}}}