{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:42:08Z","timestamp":1745469728836,"version":"3.40.4"},"reference-count":25,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>Background\/Aims: To see whether celecoxib prevents gastric cancer occurrence by disrupting the progression of chronic gastritis into gastric carcinoma through its inhibition of the migration of CD133-positive cells, one of the surface markers of bone marrow-derived cells, in Helicobacter pylori-infected gerbils. Methods: 70 gerbils were divided into six groups. Group 1 gerbils served as control (n = 6). 10 gerbils were given N-methyl-N-nitrosourea (MNU), 30 ppm (group 2). 6 short-term Helicobacter pylori-infected gerbils (group 3) were sacrificed after 8 weeks of H. pylori infection and 6 long-term H. pylori-infected gerbils were sacrificed after 42 weeks of H. pylori infection (group 4). 20 gerbils were given MNU pretreatment and long-term H. pylori infection (group 5). In addition, after H. pylori inoculation, 22 gerbils also received a celecoxib in their diet (group 6). CD133 and CCR2 expression in gastric tissues was evaluated by Western blot analysis and immunostaining. Results: CD133-positive cells were mainly localized in the bottom of the gastric epithelial cells. CD133-positive cells also migrated into gastric cancer tissues in this model. CD133-positive cells in MNU-pretreated H. pylori-infected gerbils were significantly increased compared to those in H. pylori short-term infected gerbils. Celecoxib treatment significantly reduced CD133-positive cell migration and CCR2 expression levels. CD133- and CCR2-positive cells were colocalized in H. pylori-infected gastritis and gastric cancer tissues. Celecoxib treatment significantly reduced the number of CD133- and CCR2-positive cells. Conclusions: Celecoxib inhibits CD133-positive cell migration via the reduction of CCR2 in this model. Further studies are needed to clarify the precise mechanisms driving H. pylori infection-induced CD133-positive cell migration and its link to the progression of chronic gastritis into gastric cancer.<\/jats:p>","DOI":"10.1159\/000252790","type":"journal-article","created":{"date-parts":[[2010,1,18]],"date-time":"2010-01-18T13:14:48Z","timestamp":1263820488000},"page":"193-203","source":"Crossref","is-referenced-by-count":6,"title":["Celecoxib Inhibits CD133-Positive Cell Migration via Reduction of CCR2 in Helicobacter pylori-Infected Mongolian Gerbils"],"prefix":"10.1159","volume":"81","author":[{"given":"Seiji","family":"Futagami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuhiko","family":"Hamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Shimpuku","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Nagoya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Kawagoe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akane","family":"Horie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomotaka","family":"Shindo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katya","family":"Gudis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Choitsu","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,1,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0016-5085%2898%2970581-5"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2801%2900328-2"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature00870"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0301-472X%2800%2900635-4"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2F86439"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1634%2Fstemcells.2007-0054"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1099513"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1158%2F0008-5472.CAN-05-2018"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature05372"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1093%2Fcarcin%2F20.4.669"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1523-5378.2005.00305.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1749-6632.2003.tb03242.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.biocel.2004.08.010"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjc.6604437"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1634%2Fstemcells.2007-0163"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1634%2Fstemcells.2007-0409"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1097%2FSLA.0b013e31816401d5"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1078%2F0171-9335-00403"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1523%2FJNEUROSCI.3016-07.2007"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.stem.2008.03.003"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M804220200"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1136%2Fgut.52.9.1257"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00535-007-2151-8"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1440-1746.2007.04866.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1136%2Fgut.2003.021477"}],"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/dig\/article-pdf\/81\/3\/193\/4033896\/000252790.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/dig\/article-pdf\/81\/3\/193\/4033896\/000252790.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T21:01:22Z","timestamp":1745442082000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000252790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":25,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2010,2,1]]},"published-print":{"date-parts":[[2010,2,2]]}},"URL":"https:\/\/doi.org\/10.1159\/000252790","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"type":"print","value":"0012-2823"},{"type":"electronic","value":"1421-9867"}],"subject":[],"published":{"date-parts":[[2010]]}}}