{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:40:27Z","timestamp":1757706027575,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2009,11,4]],"date-time":"2009-11-04T00:00:00Z","timestamp":1257292800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,11,4]],"date-time":"2009-11-04T00:00:00Z","timestamp":1257292800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Nephrol"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Epithelial-mesenchymal transition (EMT) is important in the development of peritoneal fibrosis. Glucose degradation products (GDPs) may induce EMT in human peritoneal mesothelial cells (HPMCs). &lt;i&gt;Methods:&lt;\/i&gt; The effects of individual GDPs and GDPs derived from peritoneal dialysis fluid (PDF) in both HPMCs and peritoneal membranes were evaluated. EMT was assessed with \u03b1-smooth muscle actin (\u03b1-SMA) and E-cadherin. &lt;i&gt;Results:&lt;\/i&gt; In vitro, \u03b1-SMA protein and mRNA levels increased in the presence of the GDPs (formaldehyde, glyoxal, methylglyoxal, and 3-deoxyglucosone), and E-cadherin decreased. Changes in the EMT markers were most prominent after exposure to 3-deoxyglucosone. Changes in both \u03b1-SMA and E-cadherin protein levels were less with low (L)-GDP bicarbonate\/lactate-buffered PDF compared to high (H)-GDP PDF. In the rat model after 8 weeks\u2019 PDF infusion, the \u03b1-SMA\/E-cadherin mRNA ratio increased in the H-GDP group compared with the L-GDP group (p &lt; 0.05). The peritoneum in the H-GDP group tended to be thicker (p = 0.052) and had more blood vessels than that in the L-GDP group (p &lt; 0.05). Tissue staining for TGF-\u03b21 decreased in the L-GDP group. Dual-stained cytokeratin and \u03b1-SMA-positive myofibroblasts in the submesothelial layer were more prominent in the H-GDP group. &lt;i&gt;Conclusion:&lt;\/i&gt; GDPs found in PDF induce EMT of HPMCs, which is associated with peritoneal fibrosis and vascularization. Conversely, L-GDP PDF reduces EMT and peritoneal fibrosis.<\/jats:p>","DOI":"10.1159\/000256658","type":"journal-article","created":{"date-parts":[[2009,11,6]],"date-time":"2009-11-06T11:08:48Z","timestamp":1257505728000},"page":"58-67","source":"Crossref","is-referenced-by-count":23,"title":["Impact of Low Glucose Degradation Product Bicarbonate\/Lactate-Buffered Dialysis Solution on the Epithelial-Mesenchymal Transition of Peritoneum"],"prefix":"10.1159","volume":"31","author":[{"given":"Eun-Joo","family":"Oh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye-Myung","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soon-Youn","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju-Min","family":"Yook","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan-Duck","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Hee","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho-Young","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In-San","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-A","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duk-Hee","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Lim","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,11,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1242%2Fdev.02141"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa020809"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2004060436"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1053%2Fj.ajkd.2005.08.011"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfn495"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2FS0014-5793%2899%2901642-7"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1744-9987.2007.00431.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1990.150"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2006111292"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfl271"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.ki.5002016"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2002.00490.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfl355"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2Fnm888"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2003090795"}],"container-title":["American Journal of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/256658","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/256658","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T21:07:28Z","timestamp":1745442448000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000256658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,4]]},"references-count":15,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2010,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000256658","archive":["Portico"],"relation":{},"ISSN":["0250-8095","1421-9670"],"issn-type":[{"type":"print","value":"0250-8095"},{"type":"electronic","value":"1421-9670"}],"subject":[],"published":{"date-parts":[[2009,11,4]]}}}