{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:08:13Z","timestamp":1788350893002,"version":"build-2803163510"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,11,18]],"date-time":"2009-11-18T00:00:00Z","timestamp":1258502400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,11,18]],"date-time":"2009-11-18T00:00:00Z","timestamp":1258502400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Caries Res"],"abstract":"<jats:p>The influence of salivary pellicles on the demineralization of human dental enamel by 1% citric acid was studied. The pellicles were formed on embedded human tooth surfaces incubated in vitro with various solutions for periods of up to 6 days. Pellicles induced by unstimulated whole saliva gave an approximately 45% and those from parotid saliva a 25% inhibition of demineralization. On the other hand, a pellicle formed from mixed submandibular and sublingual (SM-SL) saliva in 30 min gave a retardation in lesion formation of at least 40% and after a 60-min exposure to SM-SL saliva, the lesion formation was completely prevented. In contrast, when the mucins were removed from SM-SL saliva by ultracentrifugation, protection against demineralization by the pellicle formed on exposure to the supernatant was only 30%. The effect of pellicles obtained by isolated salivary mucins was also studied. Human whole salivary mucins (HWSM) were isolated from both human SM-SL saliva and whole saliva of two individuals with blood group A. The mucins contained 18% protein, 72% carbohydrate, 1.4% sulfate and 0.14% phosphate. The major components of the protein moiety were threonine (14.7%), valine (12.6%) and serine (10.8%). The molar ratio of the sugar residues was: galactose: N-acetylglucosamine: N-acetylgalactose: fucose: N-acetylneuraminic acid = 6:4.5:4:7:1. A pellicle formed by a 3-day exposure to salivary mucins in vitro gave 100% protection of the tooth surface against demineralization by citric acid. These data suggest that the mucins in human saliva contribute to a large extent to its protective effect against acidic attacks on the tooth surface.<\/jats:p>","DOI":"10.1159\/000261033","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T03:28:15Z","timestamp":1259033295000},"page":"297-309","source":"Crossref","is-referenced-by-count":84,"title":["Role of Mucins from Human Whole Saliva in the Protection of Tooth Enamel against Demineralization in vitro"],"prefix":"10.1159","author":[{"given":"Nieuw","family":"Amerongen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.H.","family":"Oderkerk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.A.","family":"Driessen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2009,11,18]]},"container-title":["Caries Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000261033","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:50:33Z","timestamp":1777481433000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000261033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,18]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000261033","archive":["Portico"],"relation":{},"ISSN":["0008-6568","1421-976X"],"issn-type":[{"value":"0008-6568","type":"print"},{"value":"1421-976X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11,18]]}}}