{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T00:24:36Z","timestamp":1726187076961},"reference-count":24,"publisher":"S. Karger AG","issue":"Suppl. 1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ORL"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>&lt;i&gt;Objective:&lt;\/i&gt;Water homeostasis is essential for the inner ear to maintain the function of hearing and equilibrium. Bumetanide inhibits Na&lt;sup&gt;+&lt;\/sup&gt;-K&lt;sup&gt;+&lt;\/sup&gt;-2Cl&lt;sup&gt;\u2013&lt;\/sup&gt; co-transporter (NKCC), which is expressed in the basolateral membrane of stria vascularis marginal cell, and it causes the enlargement of intrastrial space. Aquaporin (AQP) 2 is expressed in the perilymph side of stria vascularis basal cell, and the vasopressin type 2 antagonist OPC-31260 produces downregulation of AQP2 mRNA levels in the inner ear. The aim of this study is to investigate the influence of OPC-31260 on experimentally induced enlargement of the intrastrial space caused by bumetanide. &lt;i&gt;Materials and Methods:&lt;\/i&gt; Wistar rats were divided into two groups, a BUM group and an OPC-BUM group. The BUM group was exposed to bumetanide, and the OPC-BUM group was exposed to bumetanide after being premedicated with OPC-31260. The specimens of the stria vascularis were observed using transmission electron microscopy and analyzed quantitatively and statistically. &lt;i&gt;Results:&lt;\/i&gt; Morphological changes of intrastrial space enlargement occurred in both the BUM and OPC-BUM groups. The ratio of the areas of the intrastrial space area to the stria vascularis were calculated, and the OPC-BUM group mean showed a minimal difference from the BUM-group mean. However, there is no statistical difference. &lt;i&gt;Conclusions:&lt;\/i&gt; Premedication of rats with OPC-31260 tended to reduce bumetanide-induced enlargement of the intrastrial space. This may indicate that the effect of bumetanide on the stria vascularis is much stronger than that of OPC-31260.<\/jats:p>","DOI":"10.1159\/000265114","type":"journal-article","created":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T09:20:39Z","timestamp":1267435239000},"page":"10-15","source":"Crossref","is-referenced-by-count":1,"title":["Bumetanide-Induced Enlargement of the Rat Intrastrial Space and Effects of a Vasopressin Type 2 Antagonist"],"prefix":"10.1159","volume":"71","author":[{"given":"Masahiko","family":"Nishimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akinobu","family":"Kakigi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taizo","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruhiko","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Doi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,2,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-3495%2800%2976497-6"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1080%2F003655402%2F000028051"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-5955%2803%2900226-0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1080%2F00016480802441754"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.anl.2008.04.012"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.256.5055.385"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-5955%2899%2900036-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097%2F00001756-200207020-00011"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-5955%2803%2900131-X"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1097%2Fmlg.0b013e318031c802"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1080%2F00016480701765691"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1007%2F978-3-540-79885-9_8"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1097%2F00001756-199904260-00009"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-5955%2899%2900180-X"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.anl.2007.12.005"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.anl.2008.05.013"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-5955%2803%2900135-7"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.heares.2006.05.001"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.pharmtox.41.1.175"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1080%2F00016480802419115"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuroscience.2004.06.045"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00186783"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004410051118"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.heares.2009.09.011"}],"container-title":["ORL"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/265114","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/265114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T22:53:22Z","timestamp":1726181602000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/ORL\/article\/doi\/10.1159\/000265114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":24,"journal-issue":{"issue":"Suppl. 1","published-online":{"date-parts":[[2010,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000265114","archive":["Portico"],"relation":{},"ISSN":["0301-1569","1423-0275"],"issn-type":[{"type":"print","value":"0301-1569"},{"type":"electronic","value":"1423-0275"}],"subject":[],"published":{"date-parts":[[2009]]}}}