{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:15:27Z","timestamp":1777943727720,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T00:00:00Z","timestamp":1260403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T00:00:00Z","timestamp":1260403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"abstract":"<jats:p>Furosemide (1 mM), a potent loop diuretic, caused a 10-mV (n = 14) depolarization of the intracellular potential difference (PD&lt;sub&gt;I&lt;\/sub&gt;) of isolated rabbit ciliary epithelium (CE), but produced a 9-mV (n = 5) hyperpolarization of PDI of isolated human CE. In rabbit CE, furosemide consistently depolarized PDi byChloride transport 13, 7 and 8 mV in HCO&lt;sub&gt;3&lt;\/sub&gt;\u2013\u2013-free Ringer, Na+-free Ringer and after BaCl&lt;sub&gt;2&lt;\/sub&gt; treatment, respectively. The depolarization of PD&lt;sub&gt;I&lt;\/sub&gt; was reduced to 2 mV (n = 11) in Cl-free conditions. A hyper polarization of PD&lt;sub&gt;I&lt;\/sub&gt; caused by furosemide that was quantitatively similar to that seen in normal Ringer also occurred in human CE during immersion in HCO&lt;sub&gt;3&lt;\/sub&gt;\u2013\u2013-free Ringer, Na+-free Ringer and after BaCl&lt;sub&gt;2&lt;\/sub&gt; treatment. There was a small hyperpolarization (3 mV) of PD&lt;sub&gt;I&lt;\/sub&gt; in Cl\u2013\u2013-free conditions. Human or rabbit tissue-cultured nonpigmented ciliary epithelial cells were loaded with the Cl\u2013\u2013-sensitive fluorophore 6-methoxy-N-(3-sul-fopropyl) quinolinium (SPQ) in hypotonic solution (145 mosm) for 4 min at 37\u00b0C. Furosemide decreased intracellular Cl\u2013\u2013 fluorescence activity of both human and rabbit ciliary epithelial cells by 30 \u00b1 5 (n = 8) and 25 \u00b1 7% (n = 13), respectively, when the cells were immersed in Ch-rich solution. It is suggested that a furosemide-sensitive Cl\u2013\u2013- movement exists in both rabbit and human CE, although the mode of Cl\u2013\u2013 movement to the aqueous across CE may differ between these species.<\/jats:p>","DOI":"10.1159\/000267151","type":"journal-article","created":{"date-parts":[[2009,12,11]],"date-time":"2009-12-11T02:44:11Z","timestamp":1260499451000},"page":"83-91","source":"Crossref","is-referenced-by-count":7,"title":["Comparative Studies of Furosemide Effects on Membrane Potential and Intracellular Chloride Activity in Human and Rabbit Ciliary Epithelium"],"prefix":"10.1159","volume":"24","author":[{"given":"T.C.","family":"Chu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.R.","family":"Socci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Coca-Prados","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,12,10]]},"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000267151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T07:34:27Z","timestamp":1777707267000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000267151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,10]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1992,2,1]]},"published-print":{"date-parts":[[1992,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000267151","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"value":"0030-3747","type":"print"},{"value":"1423-0259","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,10]]}}}