{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:24:02Z","timestamp":1777890242570,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T00:00:00Z","timestamp":1261526400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T00:00:00Z","timestamp":1261526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gyn\u00e4kol Geburtshilfliche Rundsch"],"abstract":"<jats:p>Fragestellung: Umschriebene chromosomale Allelverluste (= loss of heterozygosity = LOH) sind ein starker Hinweis auf pathogenetisch bedeutsame Tumorsuppressorgene in den betroffenen Chromosomenregionen. In der vorliegenden Studie haben wir sporadische Mammakarzinome auf verschiedene LOH\u2019s hin untersucht. Material und Methode: Es wurden 47 Karzinome mit Mikrosatellitenmarkern auf das Vorliegen von LOH\u2019s hin analysiert. Dabei wurden 18 Loci auf 7 verschiedene Chromosomen ausgew\u00e4hlt. Nach PCR-Amplifikation wurden die DNA-Fragemente aus Tumor- und Normalgewebe der Patientinnen durch 8% denaturierende Polyarcylamidgelelektrophorese (PAGE) separiert und mit Silberf\u00e4rbung sichtbar gemacht. Ergebnisse: F\u00fcr die einzelnen Loci fanden wir folgende LOH-Raten: D6S497 (Chromosom 6p21, WAF-Region): 0%, D7S495: 9%, D7S522: 13%, D7S523: 22%, D11S488 (11q24-25): 38%, D13S321 bzw. D13S765 (13q13-14, Rb-Region): 30% bzw. 17%, D13S260 bzw. D13S267 (13q12.3, BRCA2-Region): 28% beide, D16S539 (16q22-24, E-Cadherin-Region): 35%, D17S5 (17p13.3): 17%, TP53 (17p13.1): 32% D17S250 (17q11-12): 22%, D17S855 (17q21 innerhalb des BRCAl-Gens): 25%, D17S579 (17q21 telomer von BRCA1): 13%, D17S846 (centromer von BRCA1): 17%, 17q24 (SSTR 2): 9%, D22S684 (22q12, NF2-Region): 20%. Insgesamt hatten 66% aller Tumoren Allelverluste. In lymphknotenpositiven Tumoren waren LOH\u2019s signifikant h\u00e4ufiger nachweisbar. Schlu\u03b2folgerung: Die h\u00f6chsten LOH-Raten auf den Chromosomen 11, 13, 16 und 17 deuten auf relevante TSG in den untersuchten Bereichen hin. Das Auftreten von LOH\u2019s in den hier untersuchten Loci ist als prognostisch ung\u00fcnstiger Marker zu werten.<\/jats:p>","DOI":"10.1159\/000272566","type":"journal-article","created":{"date-parts":[[2009,12,24]],"date-time":"2009-12-24T03:16:40Z","timestamp":1261624600000},"page":"63-67","source":"Crossref","is-referenced-by-count":2,"title":["Nachweis genetischer Alterationen in sporadischen Brusttumoren"],"prefix":"10.1159","author":[{"given":"Rita Karin","family":"Schmutzler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Homann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Bierhoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.D.","family":"Wiestler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"von Daimling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Krebs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2009,12,23]]},"container-title":["Gyn\u00e4kologisch-geburtshilfliche Rundschau"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000272566","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:34:00Z","timestamp":1777613640000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000272566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,23]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000272566","archive":["Portico"],"relation":{},"ISSN":["1018-8843","1423-0011"],"issn-type":[{"value":"1018-8843","type":"print"},{"value":"1423-0011","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,23]]}}}