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Karger AG","issue":"5","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Audiol Neurotol"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>Studies conducted over the last few years demonstrated that signaling pathways that operate in the organs of Corti (OC) play a central role in survival and death of hair cells. An important goal of molecular otology is to characterize these signaling pathways in normal inner ears and inner ears exposed to a variety of different forms of stress, such as ototoxic substances and noise overexposure. In this study, we used high-performance reverse protein microarray technology and phospho-specific antibodies to examine the activation status of defined molecules involved in cellular signaling. We demonstrate that reverse protein microarrays based on the highly sensitive planar-waveguide technology provide an effective and high-throughput means to assess the activation state of key molecules involved in apoptotic and prosurvival signaling in microdissected OC explants over time. In this study, we show that gentamicin and a specific NF-\u0138B inhibitor increase the ratio of phospho-c-Jun\/c-Jun in OC explants of postnatal rats soon after exposure to these drugs. In addition, we found a decrease in the phospho-Akt\/Akt ratio in OC explants early after NF-\u0138B inhibition. Finally, we observed an early and consistent decrease in the phospho-p38\/p38 ratio in OC explants exposed to the NF-\u0138B inhibitor and only a transient decrease in this ratio in OC examples after gentamicin exposure.<\/jats:p>","DOI":"10.1159\/000279760","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T10:55:56Z","timestamp":1265194556000},"page":"282-290","source":"Crossref","is-referenced-by-count":11,"title":["Stress and Survival Pathways in the Mammalian Cochlea"],"prefix":"10.1159","volume":"15","author":[{"given":"Antje","family":"Caelers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vesna","family":"Radojevic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Traenkle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves","family":"Brand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Bodmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,2,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuroscience.2005.10.010"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuroscience.2003.08.041"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1055%2Fs-2002-36100"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-5955%2802%2900514-2"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002%2Flary.20058"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097%2F01.moo.0000186799.45377.63"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuroscience.2009.02.085"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10162-006-0050-y"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0736-5748%2899%2900094-5"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.cdd.4401706"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002%2Fjnr.20392"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002%2Fjnr.20833"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1471-4159.2006.04117.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1055%2Fs-2004-814280"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2Fneu.20054"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1159%2F000101328"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10162-005-0006-7"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.anl.2004.11.006"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002%2F1615-9861%28200204%292%3A4%3C383%3A%3AAID-PROT383%3E3.0.CO%3B2-E"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2F0006-2952%2895%2902160-4"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038%2F356397a0"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2F0378-5955%2891%2990062-E"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2FS1359-6446%2805%2903552-X"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2FS0378-5955%2801%2900224-6"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.76.9.4350"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016%2FS0167-7799%2801%2901910-2"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuroscience.2004.11.014"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1002%2Fpmic.200500078"}],"container-title":["Audiology and Neurotology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/279760","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/279760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T21:14:38Z","timestamp":1745442878000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000279760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":28,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2010,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000279760","archive":["Portico"],"relation":{},"ISSN":["1420-3030","1421-9700"],"issn-type":[{"type":"print","value":"1420-3030"},{"type":"electronic","value":"1421-9700"}],"subject":[],"published":{"date-parts":[[2010]]}}}