{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:04:08Z","timestamp":1791173048596,"version":"4.1.0"},"reference-count":16,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>&lt;i&gt;Tannerella forsythia&lt;\/i&gt; is a gram-negative bacterium strongly associated with the development and\/or progression of periodontal disease. Here, we have shown that a newly characterized matrix metalloprotease-like enzyme, referred to as karilysin, efficiently cleaved the antimicrobial peptide LL-37, significantly reducing its bactericidal activity. This may contribute to the resistance of &lt;i&gt;T. forsythia&lt;\/i&gt; to the antibacterial activity of LL-37, since their vitality was found not to be affected by LL-37 at concentrations up to 2.2 \u00b5&lt;i&gt;M&lt;\/i&gt;. Furthermore, proteolysis of LL-37 by karilysin not only abolished its ability to bind lipopolysaccharide (LPS) to quench endotoxin-induced proinflammatory activity, but LL-37 cleavage also caused the release of active endotoxin from the LPS\/LL-37 complex. Proteolytic inactivation of LL-37 bactericidal activity by karilysin may protect LL-37-sensitive species in the subgingival plaque and maintain the local inflammatory reaction driven by LPS from gram-negative bacteria. Consequently, the karilysin protease may directly contribute to periodontal tissue damage and the development and\/or progression of chronic periodontitis.<\/jats:p>","DOI":"10.1159\/000281881","type":"journal-article","created":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T15:32:14Z","timestamp":1265297534000},"page":"288-293","source":"Crossref","is-referenced-by-count":59,"title":["Proteolytic Inactivation of LL-37 by Karilysin, a Novel Virulence Mechanism of &lt;i&gt;Tannerella forsythia&lt;\/i&gt;"],"prefix":"10.1159","volume":"2","author":[{"given":"Joanna","family":"Koziel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aabdulkarim Y.","family":"Karim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornelia","family":"Przybyszewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslaw","family":"Ksiazek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Rapala-Kozik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ky-Anh","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Potempa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2010,2,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038%2Fnbt1267"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000226256"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159%2F000171533"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038%2Fnbt1288"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2249.2006.03200.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1902%2Fannals.1999.4.1.32"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2802%2911201-3"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1099%2F13500872-141-4-921"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1515%2FBC.2010.009"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000181144"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1371%2Fjournal.pone.0005210"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1399-302X.2008.00433.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159%2F000181015"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdki103"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1515%2FBC.2009.037"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2958.2002.03146.x"}],"container-title":["Journal of Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/281881","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/281881","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T20:53:01Z","timestamp":1745441581000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000281881"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":16,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2010,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000281881","archive":["Portico"],"relation":{},"ISSN":["1662-811X","1662-8128"],"issn-type":[{"value":"1662-811X","type":"print"},{"value":"1662-8128","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}