{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:46:08Z","timestamp":1777891568849,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T00:00:00Z","timestamp":1267142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T00:00:00Z","timestamp":1267142400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"abstract":"<jats:p>The effects of oxytocin, PGF&lt;sub&gt;2\u03b1&lt;\/sub&gt; and PGE&lt;sub&gt;2&lt;\/sub&gt; on [Ca2+]&lt;sub&gt;i&lt;\/sub&gt; of isolated longitudinal muscle cells of term pregnant rats were investigated using Fura-2. Oxytocin, PGF&lt;sub&gt;2\u03b1&lt;\/sub&gt; and PGE&lt;sub&gt;2&lt;\/sub&gt; induced an initial rapid increase followed by a secondary gradual increase in [Ca2+]&lt;sub&gt;i&lt;\/sub&gt; in the presence of 1.5 mM Ca2+. The initial maximum increases in [Ca2+]&lt;sub&gt;i&lt;\/sub&gt; were obtained at 6 s for oxytocin, 20 s for PGF&lt;sub&gt;2\u03b1&lt;\/sub&gt; and 30 s for PGE&lt;sub&gt;2&lt;\/sub&gt; after addition of the stimulants. The EC&lt;sub&gt;50&lt;\/sub&gt; values obtained from the curves were 2.0 nM for oxytocin, 250 nM for PGF&lt;sub&gt;2\u03b1&lt;\/sub&gt; and 2,200 nM for PGE&lt;sub&gt;2&lt;\/sub&gt;. On the other hand, the increases in [Ca2+]&lt;sub&gt;i &lt;\/sub&gt;induced by the stimulants were nearly abolished by removal of the extracellular Ca2+. The stimulants induced biphasic increases in [Ca2+]&lt;sub&gt;i&lt;\/sub&gt; which were highly extracellular Ca2+-dependent processes, and the order of potencies for the stimulants was oxytocin \u00bb PGF&lt;sub&gt;2\u03b1&lt;\/sub&gt; &gt; PGE&lt;sub&gt;2&lt;\/sub&gt; in terms of affinity as well as magnitude. These results might indicate differences in the weight of the physiological role of the stimulants for regulating uterine contractility.<\/jats:p>","DOI":"10.1159\/000291842","type":"journal-article","created":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T04:20:08Z","timestamp":1267417208000},"page":"145-149","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Oxytocin, Prostaglandin F\n                    <sub>2\u03b1<\/sub>\n                    and Prostaglandin E\n                    <sub>2<\/sub>\n                    on Intracellular Free Calcium Concentrations of Longitudinal Muscle Cells Isolated from Term Pregnant Rat Myometrium"],"prefix":"10.1159","author":[{"given":"Tatsuhiko","family":"Kawarabayashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideki","family":"Shojo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuji","family":"Tsukamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,2,26]]},"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000291842","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:06:58Z","timestamp":1777615618000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000291842"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,26]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000291842","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"value":"0378-7346","type":"print"},{"value":"1423-002X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2,26]]}}}