{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T14:27:27Z","timestamp":1758810447123,"version":"3.40.4"},"reference-count":34,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T00:00:00Z","timestamp":1277856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T00:00:00Z","timestamp":1277856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2011]]},"abstract":"<i>Background:<\/i> It has been suggested from several animal studies and clinical observations that congenital diaphragmatic hernia (CDH) with pulmonary hypoplasia is accompanied by a disturbed perinatal ion transport. This could lead to respiratory distress due to slower clearance of fetal lung fluid at birth. <i>Objectives:<\/i> The purpose of this study was to determine whether CDH is related to changes in the expression of three rate-limiting transporter proteins in lung epithelium at birth. <i>Methods:<\/i> Tracheal aspirate was collected from 12 newborn infants with CDH and from 8 newborn control patients. Sampling was performed at postnatal age 18 and at 43 h in the CDH group and at 18 h in the control group. The protein abundance of \u03b1-, \u03b2- and \u03b3-epithelial Na<sup>+<\/sup> channel (ENaC), aquaporin 5 and Na<sup>+<\/sup>, K<sup>+<\/sup>-ATPase \u03b1<sub>1<\/sub> was analyzed using semiquantitative immunoblotting. <i>Results:<\/i> The levels of \u03b2-ENaC, \u03b3-ENaC and Na<sup>+<\/sup>, K<sup>+<\/sup>-ATPase \u03b1<sub>1<\/sub> collected at 18 h postnatally were significantly lower in CDH infants compared to control infants. In the CDH group, no significant difference in the expression of the ENaC subunits, Na<sup>+<\/sup>, K<sup>+<\/sup>-ATPase \u03b1<sub>1<\/sub> or aquaporin 5 could be detected between the two sampling time points. <i>Conclusions:<\/i> This downregulation may result in an abnormal lung fluid absorption which could be an important mechanism behind the respiratory distress seen in newborn CDH patients.<\/jats:p>","DOI":"10.1159\/000292503","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T06:59:24Z","timestamp":1277881164000},"page":"14-22","source":"Crossref","is-referenced-by-count":4,"title":["Low Pulmonary Expression of Epithelial Na<sup>+<\/sup> Channel and Na<sup>+<\/sup>, K<sup>+<\/sup>-ATPase in Newborn Infants with Congenital Diaphragmatic Hernia"],"prefix":"10.1159","volume":"99","author":[{"given":"Andreas","family":"Ringman 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