{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:39:24Z","timestamp":1745469564150,"version":"3.40.4"},"reference-count":17,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Urol Int"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>&lt;i&gt;Objectives:&lt;\/i&gt; To evaluate the correlation between vitamin K-dependent gamma-glutamyl carboxylase (GGCX) and the formation of calcium oxalate urolithiasis (COU). &lt;i&gt;Methods:&lt;\/i&gt; Renal cortex samples were obtained from patients undergoing nephrectomy and divided into 3 groups: (1) urolithiasic group (n = 44); (2) hydronephrosis-without-stone group (control group A, n = 6), and (3) normal control group (control group B, n = 21). Immunohistochemical technique was established to study the location of GGCX in renal tissues. The carboxylase activity was detected by the isotope-labeled carboxylatic reaction in vitro. We also quantified GGCX mRNA levels using TaqMan real-time reverse-transcription PCR, and protein was detected by Western blot. &lt;i&gt;Results:&lt;\/i&gt; GGCX was located in the cytoplasm of renal tubular epithelial cells and the activity of GGCX in the urolithiasic group was significantly decreased compared with that of controls (p &lt; 0.05). GGCX mRNA in the urolithiasic group was lower than that in the normal control group, which was on average 7.86-fold underexpressed in the urolithiasic group compared to the normal control group. Protein expression of GGCX in the urolithiasic group (27.64 \u00b1 0.29) was weaker than that in the other 2 control groups (control group A 55.22 \u00b1 0.36, control group B 53.78 \u00b1 0.33), p &lt; 0.05. &lt;i&gt;Conclusions:&lt;\/i&gt; The activity and expression of GGCX are decreased in renal tissues of patients with COU.<\/jats:p>","DOI":"10.1159\/000300570","type":"journal-article","created":{"date-parts":[[2010,7,26]],"date-time":"2010-07-26T07:09:54Z","timestamp":1280128194000},"page":"94-99","source":"Crossref","is-referenced-by-count":4,"title":["Activity and Expression of Vitamin K-Dependent Gamma-Glutamyl Carboxylase in Patients with Calcium Oxalate Urolithiasis"],"prefix":"10.1159","volume":"85","author":[{"given":"Tao","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiankun","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhangqun","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,3,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1093%2Fqjmed%2Fhci041"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000107961"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1996.121"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1432-1327.1999.00448.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.urology.2005.07.058"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ju.0000146847.24571.c8"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007%2Fs11596-009-0514-5"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1006%2Fmeth.2001.1262"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS0076-6879%2897%2982117-9"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000019523"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2005-04-1711"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.tpj.6500313"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10038-007-0183-9"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bone.2006.08.007"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1097%2F01.mbc.0000240927.88177.d1"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1464-410X.2004.05016.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002%2Fpath.2061"}],"container-title":["Urologia Internationalis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/300570","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/300570","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T20:06:42Z","timestamp":1745438802000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000300570"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":17,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2010,7,1]]},"published-print":{"date-parts":[[2010,8,9]]}},"URL":"https:\/\/doi.org\/10.1159\/000300570","archive":["Portico"],"relation":{},"ISSN":["0042-1138","1423-0399"],"issn-type":[{"type":"print","value":"0042-1138"},{"type":"electronic","value":"1423-0399"}],"subject":[],"published":{"date-parts":[[2010]]}}}