{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T11:11:58Z","timestamp":1777893118999,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T00:00:00Z","timestamp":1268870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T00:00:00Z","timestamp":1268870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Invest"],"abstract":"<jats:p>Human malignant trophoblast cells (BeWo line) in culture were employed to investigate the early stimulation of human chorionic gonadotropin (hCG) secretion by 1 mM dibutyryl cyclic AMP and 1 mM theophylline (dbT). The earliest increase in secreted immunoreactive hCG occurred at least 3\u00bd h following addition of dbT, and was preceded by an increase in intracellular hCG. These results suggested that dbT stimulated hCG synthesis, rather than release. Addition of either 0.062 \u00b5g\/ml actinomycin D or 100 \u00b5g\/ml cordycepin along with dbT, or 3 \u00b5g\/ml \u0391-amanitin 9 h prior to addition of dbT, prevented the increase in hCG secretion at 3\u00bd\u20138 h. It was concluded that synthesis of RNA (possibly messenger RNA) is required for the early stimulation of hCG secretion by dbT.<\/jats:p>","DOI":"10.1159\/000301538","type":"journal-article","created":{"date-parts":[[2010,3,19]],"date-time":"2010-03-19T07:27:05Z","timestamp":1268983625000},"page":"365-377","source":"Crossref","is-referenced-by-count":12,"title":["Early Stimulation of Human Chorionic Gonadotropin Secretion by Dibutyryl Cyclic AMP and Theophylline in Human Malignant Trophoblast Cells in vitro: Inhibition by Actinomycin D, \u03b1-Amanitin, and Cordycepin"],"prefix":"10.1159","author":[{"given":"Roland A","family":"Pattillo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert O.","family":"Hussa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,3,18]]},"container-title":["Gynecologic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/301538","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000301538","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:48:13Z","timestamp":1777618093000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000301538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,18]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000301538","archive":["Portico"],"relation":{},"ISSN":["0017-5986","2571-6581"],"issn-type":[{"value":"0017-5986","type":"print"},{"value":"2571-6581","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,18]]}}}