{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:01:58Z","timestamp":1777615318335,"version":"3.51.4"},"reference-count":17,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2010]]},"abstract":"<i>Objectives:<\/i> Successful engraftment of human T-lymphotropic virus type 1 (HTLV-1)-infected cells and a marked increase of proviral DNA loads (PVLs) in non-obese diabetic\/severe combined immunodeficient (NOD\/SCID)\/\u03b3c<sup>null<\/sup> (NOG) mice have been reported. Whether the increased PVL in transplanted mice is due to the new infection of HTLV-1 was examined. <i>Methods:<\/i> Mononuclear cells from 3 NOG mice with primary engraftment from asymptomatic HTLV-1 carriers were transplanted into a second group of NOG mice. HTLV-1 PVL, proviral integration by fluorescence in situ hybridization assay, expression of viral antigen, and T-cell clonality were analyzed. <i>Results:<\/i> The PVLs in the secondarily transplanted NOG mice were significantly higher than those of primarily transplanted NOG mice. Multiple signals of HTLV-1 proviruses in the nucleus of the infected cells were revealed by fluorescence in situ hybridization analysis. Expression of HTLV-1<i> tax\/rex<\/i> mRNA and antigen was observed. The variety of T-cell clones was limited in the transplanted NOG mice. <i>Conclusions:<\/i> Multiple proviral integrations were considered to be due to the new infection from HTLV-1-infected cells to the other cells. Only a certain fraction of T cells seemed to have selectively survived in NOG mice after engraftment.<\/jats:p>","DOI":"10.1159\/000302760","type":"journal-article","created":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T07:11:08Z","timestamp":1269933068000},"page":"229-239","source":"Crossref","is-referenced-by-count":7,"title":["Multiple Integrations of Human T-Lymphotropic Virus Type 1 Proviruses in the Engrafted Cells from the Asymptomatic Carriers in NOD\/SCID\/\u03b3c<sup>null<\/sup> Mice"],"prefix":"10.1159","volume":"53","author":[{"given":"Ikuo","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Takajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumi","family":"Umeki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Morishita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kinta","family":"Hatakeyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Kataoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Nomura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Okayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,3,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.79.6.2031"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2886%2991298-5"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2885%2992734-5"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.2910400607"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1423-0410.1984.tb00083.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1201906"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1208974"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI8583"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fijc.22972"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1097%2F00019606-199506000-00006"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2F0165-4608%2893%2990175-L"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159%2F000065872"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.1994.tb05040.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.leu.2403829"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02983552"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.onc.1208978"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.immunol.19.1.475"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/302760","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/302760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T20:09:58Z","timestamp":1745438998000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000302760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":17,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2010,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000302760","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"value":"0300-5526","type":"print"},{"value":"1423-0100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}