{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T19:51:51Z","timestamp":1774554711660,"version":"3.50.1"},"reference-count":18,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[2010]]},"abstract":"<i>Objective:<\/i> Group B <i>Streptococcus<\/i> (GBS) and <i>Escherichia<\/i><i>coli<\/i><i>(E. coli)<\/i> are the leading causes of early-onset neonatal disease (EOD). Intrapartum antibiotic prophylaxis of GBS-colonized women decreases vertical transmission and EOD due to GBS. Nevertheless, no intervention has been developed to reduce the risk of EOD related to <i>E. coli<\/i>. Timely and accurate identification of colonized mothers is necessary to implement preventive strategies against neonatal sepsis. To screen for colonization during labor, we developed a real-time PCR assay for the simultaneous detection of GBS and neonatal invasive strains of <i>E. coli<\/i>. <i>Study Design:<\/i> Specific DNA targets for GBS are publicly available. For neonatal invasive <i>E. coli<\/i>, we analyzed candidate DNA targets by DNA hybridization on microarrays of invasive strains isolated from neonatal <i>E. coli<\/i> sepsis or meningitis (K1 and not K1 \u2018invasive\u2019 serotypes). Specificity of DNA probes was tested against a panel of bacteria and by simulating clinical conditions (spiking vaginal samples from pregnant women). Then, the characteristics of the selected probes were evaluated in a pilot study including 200 women in labor. <i>Results:<\/i> Prevalence of rectovaginal GBS and of vaginal and cervical <i>E. coli<\/i>serotype K1 colonization were 16.0, 3.5 and 3.5% by culture and 27, 10 and 8.5% by PCR, respectively. The prevalence of other invasive <i>E. coli<\/i> in the vagina and in the cervix, detected by PCR, was around 10%. Compared to the culture, considered as the gold standard, the sensitivities of the PCRs for the GBS and <i>E. coli<\/i> K1 were 97 and 71%, respectively. Specificities were 86 and 92%, respectively. Specificity is difficult to interpret, as a false-positive PCR result may in fact be a false-negative result of the culture. The turnaround time needed for PCR analysis was 2.5 h, compared to a minimum of 48 h for the culture. <i>Conclusion:<\/i> Our rapid PCR is reliable in detecting GBS in women in labor. Optimization of the PCR for invasive <i>E. coli<\/i> is needed before its implementation in clinical practice. More efforts to fight against main causes of neonatal sepsis need to be undertaken to prevent this terrible medical complication.<\/jats:p>","DOI":"10.1159\/000314014","type":"journal-article","created":{"date-parts":[[2010,10,18]],"date-time":"2010-10-18T07:41:41Z","timestamp":1287387701000},"page":"250-255","source":"Crossref","is-referenced-by-count":16,"title":["Development of a Rapid PCR Assay for Screening of Maternal Colonization by Group B <i>Streptococcus<\/i> and Neonatal Invasive <i>Escherichia coli<\/i> during Labor"],"prefix":"10.1159","volume":"70","author":[{"given":"Bego\u00f1a","family":"Mart\u00ednez de Tejada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalin M.","family":"Stan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Boulvain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gesuele","family":"Renzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Fran\u00e7ois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Irion","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Schrenzel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,10,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa020205"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1001%2Fjama.287.9.1106"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1001%2Fjama.299.17.2056"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1542%2Fpeds.108.5.1094"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa012657"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1086%2F318086"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2875%2992496-4"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM200007203430303"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1007%2Fs100960000446"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1139%2Fm92-115"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.43.3.1024-1031.2005"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.43.7.3346-3355.2005"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1542%2Fpeds.105.1.21"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ajog.2004.12.031"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2FS0306-5456%2800%2900201-1"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1542%2Fpeds.110.3.471"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2FS0029-7844%2801%2901401-6"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.41.1.254-260.2003"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/314014","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/314014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:16:42Z","timestamp":1745435802000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000314014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":18,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2010,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000314014","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"value":"0378-7346","type":"print"},{"value":"1423-002X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}