{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:47:06Z","timestamp":1773283626815,"version":"3.50.1"},"reference-count":21,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2010]]},"abstract":"<i>Background:<\/i> An increased frequency of extended-spectrum \u03b2-lactamase (ESBL)-positive <i>Proteus mirabilis<\/i> isolates was observed recently in the Clinical Hospital Center Split in Croatia. The aim of this study was the molecular characterization of ESBLs in <i>P. mirabilis<\/i> isolates from this hospital. <i>Material and Methods:<\/i> Seven strains showing reduced susceptibility to ceftazidime were investigated. Antimicrobial susceptibility was determined using the broth microdilution method. ESBLs were characterized by PCR and sequencing of <i>bla<\/i><sub>ESBL<\/sub> genes. Quinolone resistance determinants (qnr genes) were characterized by PCR. Genotyping of strains was performed by pulsed-field gel electrophoresis (PFGE). <i>Results:<\/i> The presence of an ESBL was confirmed in all strains by a double-disk synergy test. All strains were resistant to amoxicillin, piperacillin, gentamicin, ciprofloxacin, chloramphenicol, sulfamethoxazole and trimethoprim, but susceptible to ceftazidime\/clavulanic acid, piperacillin\/tazobactam, cefoxitin, imipenem and meropenem; PCR sequencing using primers targeting <i>bla<\/i><sub><i>ESBL<\/i><\/sub> genes revealed TEM-52 \u03b2-lactamase. PFGE genotyping demonstrated the clonal relatedness of TEM-52-producing <i>P. mirabilis<\/i> strains isolated from different clinical samples and wards within the hospital. <i>Bla<\/i><sub><i>TEM-52<\/i><\/sub> in 3 isolates was carried by a 70-kb conjugative plasmid. <i>Conclusions:<\/i> Our findings indicate the emergence of the TEM-52 enzyme among <i>P. mirabilis<\/i> in Croatia.<\/jats:p>","DOI":"10.1159\/000316332","type":"journal-article","created":{"date-parts":[[2010,6,11]],"date-time":"2010-06-11T07:13:15Z","timestamp":1276240395000},"page":"208-213","source":"Crossref","is-referenced-by-count":9,"title":["Emergence of <i>Proteus mirabilis<\/i> Isolates Producing TEM-52 Extended-Spectrum \u03b2-Lactamases in Croatia"],"prefix":"10.1159","volume":"56","author":[{"given":"Sanda","family":"Sardeli\u0107","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Branka","family":"Bedeni\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dubravko","family":"\u0160ijak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celine","family":"Colinon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smilja","family":"Kaleni\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,6,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02390681"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01690090"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ijid.2008.05.1196"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01690097"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkh424"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.42.6.2523-2529.2004"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1469-0691.2002.00401.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.45.4.1151-1161.2001"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2FS1473-3099%2806%2970599-0"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.01647-05"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkh045"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.39.2.791-793.2001"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkg385"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.01514-06"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.43.8.3824-3828.2005"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.40.4.1549-1552.2002"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-0691.2005.01147.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.46.3.925-928.2002"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159%2F000224657"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1159%2F000224656"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F40.3.365"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/316332","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/316332","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:38:36Z","timestamp":1745437116000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000316332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":21,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2010,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000316332","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"value":"0009-3157","type":"print"},{"value":"1421-9794","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}