{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T20:49:27Z","timestamp":1764276567798,"version":"3.40.4"},"reference-count":29,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2010]]},"abstract":"<i>Objective and Design:<\/i> Although proteinase-activated receptor (PAR)-4 has been implicated in inflammation, its role in regulating eosinophil recruitment in response to chemoattractants has not yet been demonstrated. To investigate the contribution of proteinases and PAR-4 activation to eosinophil migration in response to eotaxin-1 or leukotriene B<sub>4<\/sub> (LTB<sub>4<\/sub>), the effects of aprotinin or PAR-4 antagonist trans-cinnamoyl-YPGKF-NH<sub>2<\/sub> (tcY-NH<sub>2<\/sub>) on eosinophil migration induced by these chemoattractants were investigated. <i>Methods:<\/i> BALB\/c mice were pretreated with aprotinin or tcY-NH<sub>2<\/sub> (30 \u00b5g\/mouse) prior to intrapleural injection of LTB<sub>4<\/sub> or eotaxin-1 and the number of infiltrating eosinophils was determined 48 h later. <i>Results:<\/i> Aprotinin (1 mg\/kg) inhibited eosinophil recruitment induced by eotaxin-1 (p < 0.01), but not that induced by LTB<sub>4<\/sub>. Moreover, tcY-NH<sub>2<\/sub> treatment inhibited eosinophil recruitment in response to eotaxin-1 (p < 0.01 by ANOVA\/Tukey post-test). <i>Conclusion:<\/i> These data suggest that aprotinin-inhibited proteinases participate in eosinophil migration induced by eotaxin-1 and that PAR-4 activation plays an important role in regulating this migration.<\/jats:p>","DOI":"10.1159\/000319751","type":"journal-article","created":{"date-parts":[[2010,9,13]],"date-time":"2010-09-13T10:01:01Z","timestamp":1284372061000},"page":"224-230","source":"Crossref","is-referenced-by-count":10,"title":["Blockade of Proteinase-Activated Receptor-4 Inhibits the Eosinophil Recruitment Induced by Eotaxin-1 in the Pleural Cavity of Mice"],"prefix":"10.1159","volume":"86","author":[{"given":"A.D.","family":"Braga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.P.","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.M.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.P.","family":"Bilheiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.D.","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.N.","family":"Francischi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,9,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2008.02958.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2133.2001.04456.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159%2F000101398"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2FS0165-6147%2800%2989092-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI119690"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.lfs.2008.06.004"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.2500%2F108854198778557926"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2FS0091-6749%2898%2970056-9"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1124%2Fpr.54.2.203"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1538-7836.2005.01377.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1210%2Fer.2003-0025"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M312090200"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M513138200"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.275.10.6819"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2Fart.24300"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0706371"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2007-02-075440"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1742-1241.2008.01899.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0701122"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0707507"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.20071427"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1189%2Fjlb.0702351"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1159%2F000080037"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2007.11.025"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1002%2Fart.24300"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1189%2Fjlb.0108001"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2008.02991.x"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.icb.7100085"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.coph.2005.03.001"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/319751","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/319751","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:44:49Z","timestamp":1745437489000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000319751"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2010,10,1]]},"published-print":{"date-parts":[[2010,10,28]]}},"URL":"https:\/\/doi.org\/10.1159\/000319751","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"type":"print","value":"0031-7012"},{"type":"electronic","value":"1423-0313"}],"subject":[],"published":{"date-parts":[[2010]]}}}