{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:05:23Z","timestamp":1770483923787,"version":"3.49.0"},"reference-count":11,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med Princ Pract"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>&lt;i&gt;Objective:&lt;\/i&gt; Our aims were to measure the level of expression of Abelson (ABL1), \u03b2-glucuronidase (GUS) and glucose-6-phosphate dehydrogenase (G6PD) genes in exfoliated urine cells from healthy and transrectal ultrasound biopsy patients with elevated prostate-specific antigen levels and\/or abnormal digital rectal examinations or urinary symptoms indicative of prostate problems, as well as in archived formalin-fixed paraffin-embedded prostate materials. &lt;i&gt;Materials&lt;\/i&gt;&lt;i&gt;and Methods:&lt;\/i&gt; Real-time quantitative polymerase chain reaction (RQ-PCR) was used to evaluate the suitability of the 3 control genes, i.e. ABL1, GUS and G6PD, as control genes for prostate cancer cells. Exfoliated urine cells from 30 healthy males, 53 male patients, 138 cases of archived paraffin-embedded prostate tissues and 3 prostate cell lines were sampled. All cells were lysed in guanidine isothiocyanate buffer from which RNA was extracted and converted to cDNA by random hexamer priming. RQ-PCR was performed using TaqMan chemistries. &lt;i&gt;Results:&lt;\/i&gt; There was no significant difference in the level of expression for each of the 3 control genes in the cell lines. There was a significant difference in GUS transcript level between patients and healthy controls in both urine and prostate tissue sections (p &lt; 0.05). G6PD transcript numbers also differed significantly from those of GUS in the prostate cell lines and tissue sections (p &lt; 0.05). The transcript numbers of all the control genes were significantly reduced in aged samples (p &lt; 0.001). &lt;i&gt;Conclusion:&lt;\/i&gt; The ABL1 gene was the most stable control gene in both clinical specimens and cell lines. Therefore, we recommend its use to enable standardization and interlaboratory comparisons for the RQ-PCR of prostatic tumour markers.<\/jats:p>","DOI":"10.1159\/000320300","type":"journal-article","created":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T06:43:56Z","timestamp":1285742636000},"page":"433-439","source":"Crossref","is-referenced-by-count":9,"title":["Endogenous Control Genes in Prostate Cells: Evaluation of Gene Expression Using \u2018Real-Time\u2019 Quantitative Polymerase Chain Reaction"],"prefix":"10.1159","volume":"19","author":[{"given":"Emmanuel","family":"Nna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibtisam E.","family":"Tothill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linmarie","family":"Ludeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracey","family":"Bailey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2010,9,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.leu.2403136"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.leu.2403135"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159%2F000046635"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejca.2004.12.035"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Faja.2008.20"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.urology.2006.12.014"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0090-4295%2897%2900262-8"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1080%2F00313020701329757"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038%2Flabinvest.3700303"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002%2Fpros.20490"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1373%2Fclinchem.2008.112797"}],"container-title":["Medical Principles and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/320300","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/320300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:49:50Z","timestamp":1745437790000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000320300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":11,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2010,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000320300","archive":["Portico"],"relation":{},"ISSN":["1011-7571","1423-0151"],"issn-type":[{"value":"1011-7571","type":"print"},{"value":"1423-0151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}